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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

20.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.8K
RNA Splicing01:32

RNA Splicing

55.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.7K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.1K
5.1K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

6.0K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.0K

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相关实验视频

Updated: May 12, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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rMATS-cloud:在云中进行大规模的替代拼接分析.

Jenea I Adams1,2, Eric Kutschera2, Qiang Hu3

  • 1Genomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia 19104, USA.

Genomics, proteomics & bioinformatics
|May 9, 2025
PubMed
概括
此摘要是机器生成的。

rMATS-cloud提供了一个便携式解决方案,用于在任何云环境中从RNA测序 (RNA-seq) 数据中进行替代拼接分析. 该工具有效处理大型数据集,使其成为基于云的生物信息学研究的理想选择.

关键词:
另一个替代拼接方法.生物信息学是一种生物信息学.云计算是一种云计算.在RNA-seqqq.文字转录学 (Transcriptomics) 是一个学科.

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组学就是基因组学.

背景情况:

  • 基于云的生物信息学工具越来越常见,用于基因表达分析.
  • 然而,用于替代拼接分析的可访问云解决方案有限.
  • 自2014年以来,rMATS一直是量化替代拼接的维护,快速和多功能工具.

研究的目的:

  • 介绍rMATS-cloud,一个便携式版本的rMATS工作流.
  • 为了使生物医学研究在多种云环境中实现rMATS分析.
  • 评估rMATS-cloud在不同云平台上的性能.

主要方法:

  • 开发了rMATS-cloud作为一个便携式工作流.
  • 在两个RNA测序 (RNA-seq) 数据集上测试了rMATS-cloud.
  • 在三个云平台上的性能比较:Cavatica,Terra和Seqera.

主要成果:

  • rMATS-cloud证明了RNA-seq数据集的高效处理.
  • 工作流成功处理了数千个样本的数据集.
  • 在经过测试的云平台上,性能一致,表明了广泛的适用性.

结论:

  • rMATS-cloud是用于替代拼接分析的多功能和便携式工具.
  • 它非常适合在云环境中进行大规模的RNA-seq数据处理.
  • rMATS-cloud提高了基于云的替代拼接研究的可访问性.