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Updated: Jun 16, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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基于无模型机器学习的3D单分子定位显微镜

Miguel A Boland1, Jonathan P E Lightley2, Edwin Garcia2

  • 1Department of Mathematics, Imperial College, London, UK.

Journal of microscopy
|May 9, 2025
PubMed
概括

一种新的easyZloc方法使用轻量级的神经网络进行3D超分辨率显微镜. 这种方法与标准显微镜一起工作,减少对高通量单分子局部化显微镜 (SMLM) 工作流程的计算需求.

关键词:
在美国,CNN是CNN.深度学习是一种深度学习.简单的风暴.光是一种光.这是一个开放的FrameFrame.单个分子的定位定位.超高分辨率显微镜技术

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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