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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jun 16, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

基于无模型机器学习的3D单分子定位显微镜.

Miguel A Boland¹, Jonathan P E Lightley², Edwin Garcia²

¹Department of Mathematics, Imperial College, London, UK.

Journal of microscopy

|May 9, 2025

概括

此摘要是机器生成的。

一种新的easyZloc方法使用轻量级的神经网络进行3D超分辨率显微镜. 这种方法与标准显微镜一起工作,减少对高通量单分子局部化显微镜 (SMLM) 工作流程的计算需求.

关键词:

在美国,CNN是CNN.深度学习是一种深度学习.简单的风暴.光是一种光.这是一个开放的FrameFrame.单个分子的定位定位.超高分辨率显微镜技术

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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

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Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

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相关实验视频

Last Updated: Jun 16, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

科学领域:

生物物理学的生物物理.
显微镜的使用方法
计算生物学计算生物学

背景情况:

单分子定位显微镜 (SMLM) 提供超高分辨率成像,但3D SMLM通常需要显微镜修改.
现有的3D SMLM方法往往需要大量的计算资源和时间.

研究的目的:

提出适用于标准光显微镜的可访问和有效的3D SMLM方法.
为高通量3D超分辨率成像开发一种计算轻量化方法.

主要方法:

开发"easyZloc",一种使用轻量级卷积神经网络的3D SMLM技术.
将easyZloc方法应用于标准的,未经修改的光显微镜.
对生物样本的3D重建能力的演示.

主要成果:

在重建核孔综合体时,实现了与现有方法相比较的性能.
显著减少了计算能力和执行时间.
在扩展的轴距离范围内成功地进行了核外和actin样本的3D重建.

结论:

易Zloc方法为标准显微镜提供了实用和高效的3D SMLM解决方案.
这种方法适用于高通量工作流程,减少超分辨率显微镜中的计算障碍.
易Zloc扩大了生物研究中3D超分辨率成像的可访问性.