相关实验视频

Updated: May 12, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

优化MALDI矩阵及其准备用于MALDI-TOFMS分析的寡核酸

Susanne Dietrich^1,2, Anja Dollinger^1,3, Andreas Wieser^2,3,4,5

¹Chair of Analytical Chemistry, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany.

Rapid communications in mass spectrometry : RCM

|May 9, 2025

概括

通过优化矩阵选择和应用,使用矩阵辅助激光脱/电离飞行时间质谱法 (MALDI-TOF MS) 进行寡核酸分析的可重复性得到改善. 离子矩阵6-aza-2-thiothymine (ATT) 与1-methylimidazole显示出优异的性能.

科学领域:

分析化学分析化学
质谱测量质量谱测量
生物化学生物化学

背景情况:

由于矩阵选择和样本准备,通过MALDI-TOF MS进行寡核酸分析的可复制性具有挑战性.
之前的研究已经探讨了个别的参数,但缺乏统一的方法.

研究的目的:

系统地评估组合变量,以提高MALDI-TOFMS在寡核酸分析中的性能.
确定最佳的矩阵和溶剂条件,以提高质量精度和信号噪声比.

主要方法:

研究了各种矩阵,溶剂组成,添加剂和用于寡核酸分析的应用技术.
用MALDI-TOF MS在线性负模式中进行质谱分析.

主要成果:

评估了变量对质量-电荷比率和信号-噪声 (S/N) 比率标准偏差的综合影响.
在48个样本中,只有19个样本符合4-10kDa质量范围内检测的信号对噪声标准.

结论:

离子矩阵6-aza-2-thiothymine (ATT) 与1-methylimidazole连续减少了标准偏差和提高了质量精度.
3-基皮科林酸 (3-HPA) 的性能因溶剂成分和添加剂而有显著差异.

关键词:

马尔迪 - 托夫 MS 离子矩阵离子矩阵质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量氧核酸类的部分.

更多相关视频

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Published on: June 10, 2018

相关概念视频

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...