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奇托桑纳米粒子介导的dsRNA的传递,以提高Locusta migratoria中的RNAi效率

Yangyang Liu^1,2, Jianqin Zhang², Shuai Li^1,2

¹Shanxi Key Laboratory of Nucleic Acid Biopesticides, School of Synthetic Biology, Shanxi University, Taiyuan, China.

Pest management science

|May 9, 2025

概括

素纳米颗粒有效地提供双链RNA (dsRNA) 用于昆虫害虫控制. 这种绿色害虫控制策略增强了RNA干扰 (RNAi) 的效率,并通过改善dsRNA稳定性和细胞吸收,增加了昆虫死亡率.

科学领域:

生物技术是生物技术.
昆虫学昆虫学是一门学科.
材料科学材料科学材料科学

背景情况:

RNA干扰 (RNAi) 提供使用双链RNA (dsRNA) 生物杀虫剂的绿色害虫防治.
有效的dsRNA传递仍然是RNAi害虫控制应用的挑战.

研究的目的:

开发和评估基于素的纳米颗粒,以改善昆虫害虫管理中的dsrna传递.
评估素/dsRNA纳米颗粒在改善dsRNA稳定性,RNAi效率和昆虫死亡率方面的有效性.

主要方法:

基托桑纳米颗粒是用dSRNA配制的,其向的是Locusta migratoria中的LmCht10基因.
在实验室中,虫肠液中dsRNA的稳定性与裸体dsRNA和基托纳米颗粒进行了比较.
虫通过食和注射接受了奇托/dsRNA纳米颗粒的治疗,以评估RNAi效率和死亡率.

主要成果:

奇托/dsRNA纳米颗粒显著提高了虫肠液中dsRNA的稳定性.
食素/dsRNA纳米颗粒可使LmCht10转录减少67%并使死亡率增加2倍.
素/dsRNA纳米颗粒的注射实现了96.6%的RNAi效率和死亡率增加2倍,细胞吸收增强.

结论:

基于酸盐的dsRNA纳米材料提高了dsRNA稳定性,RNAi效率和昆虫死亡率.
奇托增强了表皮细胞中dsRNA的吸收,提高了沉默的有效性.
酸盐纳米材料代表了在害虫管理中推进RNAi技术的可行策略.

关键词:

龙迁徙者 (英语:Locusta migratoria) 是一种迁徙的动物.这是一种RNA干扰.基多酸盐的使用方法 dsRNA的传递方式纳米颗粒是一种纳米粒子.

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