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鉴定和描述Csa-miR159s及其表达模式在 (Cucumis sativus L.) 的不同非生物应力下

Zhenxiang Zhao^1,2, Wenhong Ao^1,2, Weirong Luo^1,2

¹School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.

Frontiers in plant science

|May 9, 2025

概括

黄瓜miR159基因通过准CsMYB基因来调节水果的膨胀和应激反应. 这项研究确定了特定的Csa-miR159功能,提供了关于黄瓜发育和非生物应激耐受性的见解.

科学领域:

植物分子生物学植物分子生物学
遗传学是一个遗传学.
农业科学农业科学

背景情况:

miR159基因家族对植物生长,发育和应激反应至关重要.
在黄瓜果实扩张和非生物应激耐受性中miR159的特定作用仍然没有特征.

研究的目的:

为了研究黄瓜Csa-miR159基因家族成员的分子特征.
阐明Csa-miR159在黄瓜果实扩张和对非生物应激反应中的作用.

主要方法:

从EnsemblPlants获得了六个Csa-miR159序列.
对Csa-miR159s.s.进行了遗传学分析.
在促进区域分析了 cis 作用的调节元件.
使用psRNA-Target预测和qRT-PCR识别了CsMYB基因.

主要成果:

遗传学分析将Csa-miR159s分为两个不同的分支.
推动者地区包含各种各样的监管要素,表明它们在增长,发展和应激反应中的作用.
通过准特定的CsMYB基因,Csa-miR159b和Csa-miR159d被确定为黄瓜果扩张的负调节者.
不同的表达模式表明了在非生物压力和植物激素反应中的潜在作用.

结论:

这项研究为黄瓜中Csa-miR159基因家族提供了宝贵的分子洞察力.
确定了Csa-miR159s作为果实扩张和非生物应激反应的关键调节者.
建立了进一步研究黄瓜中的miR159介导机制的基础.

关键词:

这就是Csa-miR159s.无生物压力是无生物压力.黄瓜 (Cucumis sativus. 黄瓜) 是一种植物. 一) 一) 一) 一) 一)果实扩张果实扩张植物激素植物激素

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