生命之树中的含铜氨基氧化酶是含铜的氨基氧化酶
Zaibao Zhang1, Tao Xiong2, Kejia Li1
1School of Life and Health Science, Huzhou College, Huzhou, Zhejiang, China.
Frontiers in plant science
|May 9, 2025
概括
含铜的氨基氧化酶 (CuAOs) 在植物和动物分化之前进化. 这项研究揭示了它们的进化历史,功能分歧和跨物种基因家族扩张.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 含铜氨氧化酶 (CuAO) 是参与聚胺氧化过程的关键酶,产生氨和过氧化.
- 植物CuAOs对压力激素有反应,而哺乳动物CuAOs (编码为AOC1-4) 与炎症等疾病有关.
- 了解CuAO的演变对于理解它们多样化的生物作用至关重要.
研究的目的:
- 阐明植物和动物中含铜氨氧化酶 (CuAO) 基因家族的进化史和功能分歧.
- 通过广泛的植物和动物基因组识别和分析CuAO正义基因.
主要方法:
- 使用HMMER和BLASTP对CuAO基因进行全基因组识别.
- 使用Muscle5和IQ-TREE2.2进行了遗传学分析.
- 使用MCScanX和CIRCOS进行合成分析.
- 整合来自不同物种的表达数据.
主要成果:
- 从 188 个植物和 79 个动物基因组中鉴定出 950 种植物和 264 种动物的 CuAO 基因组.
- 遗传学分析表明,CuAO起源于植物和动物分歧之前.
- 植物CuAOs形成两个分类,其中分类I缺乏关键活性位点动机;动物CuAOs形成三个分类 (CAO类,AOC1,AOC2-4),哺乳动物AOC分化是物种特异性的.
结论:
- CuAO基因家族起源于进化的早期,在植物中具有显著的拷贝数变异,但在动物中稳定.
- 在植物和动物的CuAOs中,明显的进化轨迹和功能专业化是显而易见的.
- 这种全基因组分析为未来对CuAO基因家族的功能研究提供了基础.
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