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表皮细胞-介质细胞竞争在肺发育和纤维化过程中协调了组织区之间的命运过渡

Kylie Klinkhammer¹, Rachel Warren¹, Joseph Knopp¹

¹Department of Medicine, Division of Pulmonary and Critical Medicine, Mayo Clinic, Rochester, MN 55905, USA.

Research square

|May 9, 2025

概括

肺部发育依赖于协调的细胞信号传递. 介质细胞Yap对抗上皮干,影响肺的修复和分化,而Snail/Slug指导纤维细胞的发育.

科学领域:

发育生物学是发展生物学.
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

组织形态发生和细胞状态转变需要精确的时间和空间协调.
肺部的发育和修复涉及复杂的细胞相互作用和信号通路.

研究的目的:

调查雅普在发育和受伤期间肺介质细胞和上皮细胞中信号传递的作用.
阐明介质因子如何影响表皮细胞状态和分化.
确定控制肺组织生成和再生中的组织规模协调的机制.

主要方法:

在小鼠模型中利用了基因操纵和血统追踪.
采用分子生物学技术来评估基因表达和蛋白质定位.
将实验数据与计算建模集成在一起,以了解组织力学.

主要成果:

介质细胞的雅普信号对抗了上皮细胞的雅普水平和茎状,影响了膜上皮细胞分化和肺部分支.
升高的介质细胞Yap加速分化,但损害了布莱米损伤后的肺修复.
介酶体的牛/虫将Yap/Taz封存起来,将脂肪生成的分化引导到膜纤维细胞1 (AF1) 上.
雅普/Myc-Tead结合促进了肌源性差异化程序.
组织规模的机械合作被认为是器官形成和再生的关键.

结论:

介质细胞亚普信号传递在肺部发育和修复中起着至关重要的,取决于环境的作用.
介质细胞和上皮细胞之间的相互作用,以及牛/牛,决定了细胞命运和组织架构.
机械力量是协调肺形态发生和再生过程的组成部分.

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