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骨髓瘤细胞衍生CCL2通过瘤相关巨细胞的积累促进肺转移

Hiroya Kondo¹, Hiroshi Tazawa^2,3, Tomohiro Fujiwara^1,4

¹Departments of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, 700-8558, Japan.

Cancer immunology, immunotherapy : CII

|May 9, 2025

概括

骨髓瘤肺转移是由CCL2驱动的,CCL2可以招募与瘤相关的巨细胞 (TAMs). 中和CCL2显著减少了肺组织中的转移和TAMs.

科学领域:

在瘤学瘤学.
癌症生物学癌症生物学
免疫学免疫学免疫学

背景情况:

骨髓瘤 (OS) 是儿童和青少年常见的一种骨癌.
肺转移显著恶化OS患者的预后.
驱动OS肺转移的机制,特别是瘤微环境 (TME),尚未完全理解.

研究的目的:

调查TME在骨髓瘤肺转移中的作用.
确定促进OS转移的关键因素.
评估CCL2作为减少OS肺转移的治疗标.

主要方法:

使用了与非转移和转移小鼠 (Dunn, LM8) 和人类 (HOS, 143B) OS细胞的 ортотоп模型.
使用细胞因子阵列和ELISA分析了TME并确定了OS细胞分泌的因子.
在体内给予CCL2中和抗体以评估治疗潜力.

主要成果:

转移性OS细胞显示M2类瘤相关巨细胞 (TAMs) 在肺组织的透增加.
转移性OS细胞通常通过细胞外囊泡分泌CCL2,部分通过细胞外囊泡.
中和CCL2抗体抑制了肺转移和M2样TAM透,但没有影响原发性瘤生长.

结论:

CCL2是促进骨髓瘤肺转移的关键因素.
CCL2通过增加肺部M2样TAM积累来促进转移.
向CCL2显示了抑制骨髓瘤肺转移的治疗潜力.

关键词:

在CCL2中,我们可以使用CCL2.细胞外囊泡细胞外囊泡肺部转移肺部转移肺部转移骨质肉瘤骨质肉瘤是什么与瘤相关的巨细胞.

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