高分辨率多色短波红外动态In Vivo用Chromenylium Nonamethine染料成像
Anthony L Spearman1, Eric Y Lin1, Emily B Mobley1
1Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Dr. East, Los Angeles, California 90095, United States.
Journal of the American Chemical Society
|May 9, 2025
概括
研究人员开发了用于先进短波红外 (SWIR) 成像的新型非甲染料. 这些明亮的,深红色的发射光体能够以前所未有的速度和细节进行高分辨率的多重化体内成像.
科学领域:
- 有机化学
- 生物医学成像
- 材料科学
背景情况:
- 短波红外 (SWIR) 成像可以快速,高分辨率的体内可视化.
- 现有的SWIR光染料通常缺乏足够的亮度或辐射超过1500nm用于深层组织成像.
- 聚甲染料为开发新型对比剂提供了多功能平台.
研究的目的:
- 合成和表征新型非甲聚甲染料,扩展到SWIR区域.
- 评估这些新染料在生物应用中的亮度和光物理特性.
- 在先进的多重体体内SWIR成像中证明这些染料的实用性.
主要方法:
- 聚甲链延伸的和染色芯.
- 吸收和发射特性的光谱表征.
- 在小鼠中使用双色和五色复合成像实验.
主要成果:
- 开发的非甲染料的吸收最大值为1149nm,排放超过1500nm.
- 在这个光谱范围内,有机光体达到最高亮度 (εmax ~ 10^5 M^-1 cm^-1, ΦF高达0.5%).
- 在小鼠中展示了高分辨率,深层组织两色SWIR成像和五色清醒成像.
结论:
- 新型非甲染料显著提升了SWIR光学成像的能力.
- 这些染料使得体内成像更明亮,更快速,更复杂,分辨率和深度更高.
- 开发的光剂代表了一代用于生物医学研究的小分子对比剂.
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