从父母细菌个体继承异常代谢活动
Yuyang Lu1, Jia Gao1, Ruo-Chen Xie1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China.
概括
细菌中强酸的产生是跨世代遗传的,但随着时间的推移逐渐减少. 这一发现对于需要可预测的细菌代谢性能的生物技术应用至关重要.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 生物技术是生物技术.
背景情况:
- 发酵等表型特征通常由于基因表达和环境而不稳定.
- 以前的研究集中在微生物群落,而不是个体细菌遗传.
- 细菌世代之间特定代谢特征的遗传仍然未得到充分研究.
研究的目的:
- 为了研究一代一代的单个 *Lactiplantibacillus plantarum* 细菌中强烈的酸产生活动的遗传性.
- 确定细菌中继承高级代谢特征的稳定性和局限性.
- 为生物技术应用提供细菌代谢特征遗传的见解.
主要方法:
- 开发了一个集成的单细胞操纵策略.
- 使用细胞外pH探针和微波阵列进行光选.
- 采用微管培养和单细胞培养来隔离和放大单个细菌.
主要成果:
- 后代细菌从母细胞中继承了强烈的酸产生活动.
- 超过30代,后代逐渐向平的活动回归.
- 环境恶化可能导致遗传特征的观察到的下降.
结论:
- 细菌的代谢特征在单细胞水平上表现出遗传特征.
- 优越特征的继承在连续几代人之间是有限的.
- 这些发现对于优化依赖于持续细菌性能的生物技术应用至关重要.
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