一个相隔的SR蛋白重编程宿主预mRNA分裂以提高疾病易感性
Dong Yan1, Jie Huang2, Fengqi Tian1
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
概括
植物病原体操纵宿主替代拼接 (AS) 来逃避免疫力. 一项研究确定了SR30,一种拼接因子,通过改变防御基因AS来抑制番茄免疫力,提供了新的育种策略.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病原体相互作用
- 在RNA分离过程中.
背景情况:
- 替代拼接 (AS) 在植物微生物相互作用中至关重要.
- 病原体利用宿主AS抑制植物免疫力.
- 宿主剪接因子调节植物免疫力的机制尚不清楚.
研究的目的:
- 确定调节免疫力的植物拼接因素.
- 研究SR30在番茄免疫力中对Phytophthora infestans的作用.
- 阐明SR30介导免疫调节的机制.
主要方法:
- 对SR30控制的mRNAAS的全基因组分析.
- 通过液-液相分离对SR30核凝聚物形成的研究.
- 在番茄中进行CRISPR/Cas9基因编辑以淘汰SR30.
- 评估SR30淘汰赛线的疾病耐药性.
主要成果:
- SR30 负面调节了番茄免疫力对抗 P. infestans. 的免疫力.
- SR30控制全基因组的mRNA AS,并抑制防御基因.
- 通过相分离,SR30通过相分离形成核凝结物,这对其功能至关重要.
- 通过促进防御基因AS,SR30淘汰赛增强了番茄对多种Phytophthora物种的抵抗力.
结论:
- SR30是番茄免疫的关键调节剂,通过AS作用.
- SR30的蛋白相分离对于其免疫调节功能至关重要.
- 针对SR30介导的AS提供了一种新的策略,用于设计抗病作物.
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