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细胞表面受体的表达克隆,用于CpG寡度氧核酸/CXCL14复合物的表达

Risa Saito¹, Manaka Hasebe², Takahiko Hara³

¹Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.

Biochemical and biophysical research communications

|May 9, 2025

概括

CpG寡氧核酸 (ODN) 激活免疫细胞,而CXCL14则增强其功能. 研究人员确定了多个细胞表面受体,包括Gpnmb,Scarb1和Igsf8,这些受体结合CpG ODN和CpG ODN/CXCL14复合体进行细胞吸收.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

众所周知,cpG寡氧核酸 (ODN) 在树突细胞和巨细胞中刺激先天免疫反应.
虽然已知一些CpG ODN受体,但CXCL14在体内CpG ODN功能中的作用突显出需要确定CpG ODN/CXCL14复合体的受体.

研究的目的:

确定参与CpG ODN和CpG ODN/CXCL14复合体的吸收的细胞表面受体分子.
阐明已识别的受体与CpG ODN或它们的复合体与CXCL14之间的特定相互作用.

主要方法:

使用来自小鼠树突细胞和巨细胞衍生细胞的cDNA库进行表达式克隆.
生物化学测试以确定已识别的分子与CpG ODN和CpG ODN/CXCL14复合体的结合亲缘关系.

主要成果:

糖蛋白非转移性黑色素瘤蛋白B (Gpnmb),食尸体受体类B1 (Scarb1) 和免疫球蛋白超级家族8 (Igsf8) 被确定为编码潜在受体的cDNA.
Gpnmb 独家绑定 CpG 的 ODNs.
Igsf8,Scarb1和Cd36结合了CpG的ODN和CpG的ODN/CXCL14复合体,而Igsf3和Rage则特别结合了Cy3-ODN2395/CXCL14复合体.

结论:

多个细胞表面受体调解CpG ODN/CXCL14复合体的细胞内吸收.
这些发现扩大了我们对CpG ODN介导免疫激活和CXCL14作用背后的分子机制的理解.

关键词:

在CXCL14中,CXCL14是最重要的.在CpG ODN中使用.克隆表达方式的克隆.这是天生的免疫力.

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