在微生物电合成中,从CO2和甲醇中选择性酸盐的生产 - - pH的影响
Hui Yao1, Meritxell Romans-Casas2, Igor Vassilev1
1Faculty of Engineering and Natural Sciences, Tampere University, Korkeakoulunkatu 8, 33720 Tampere, Finland.
Bioelectrochemistry (Amsterdam, Netherlands)
|May 9, 2025
概括
最佳的pH 6通过甲醇辅助微生物电合成 (MES) 增强了丁酸盐的产生. 这项研究探讨了pH对MES性能,微生物群落和有效的丁酸盐合成遗传潜力的影响.
科学领域:
- 环境科学 环境科学
- 生物技术是生物技术.
- 微生物学 微生物学
背景情况:
- 甲醇辅助微生物电合成 (MES) 为利用电力从二氧化碳和甲醇中产生丁酸盐提供了一条途径.
- 了解操作参数对于优化MES驱动的酸盐生产至关重要.
研究的目的:
- 为了研究pH对甲醇辅助微生物电合成 (MES) 对酸盐生产的影响.
- 为了比较不同pH值的过程性能,微生物社区结构和遗传潜力.
主要方法:
- 三个平板MES反应堆在5.5,6和7的控制pH值下在料批量模式下运行.
- 监测了工艺性能,包括酸盐选择性和生产速度.
- 用转基因组分析来研究微生物社区结构和遗传潜力.
主要成果:
- 酸盐的最高选择性 (87%) 和生产率 (0.3 g L-1 d-1) 在pH 6.0下实现.
- pH 7产生了类似的生产率,但选择性较低 (70%),与乙酸联合生产.
- 甲醇和二氧化碳的消耗量随着pH值的增加而增加,以及更多的负潜力,表明热力学可行性发生了变化.
结论:
- 在甲醇辅助MES中,pH 6是最大限度地提高酸盐选择性和生产率的最佳值.
- *Eubacterium callanderi*占据了微生物群落的主导地位,利用Wood-Ljungdahl途径进行碳同化,并可能通过逆β氧化产生丁酸盐.
相关概念视频
01:20Fates of Pyruvate
8.0K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.0K
03:08Hydroboration-Oxidation of Alkenes
7.6K
In addition to the oxymercuration–demercuration method, which converts the alkenes to alcohols with Markovnikov orientation, a complementary hydroboration-oxidation method yields the anti-Markovnikov product. The hydroboration reaction, discovered in 1959 by H.C. Brown, involves the addition of a B–H bond of borane to an alkene giving an organoborane intermediate. The oxidation of this intermediate with basic hydrogen peroxide forms an alcohol.
7.6K
01:35Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives
1.9K
Just like β-keto acids—which upon thermal decarboxylation form ketones—β-dicarboxylic acids undergo decarboxylation to generate monocarboxylic acids with the liberation of carbon dioxide.
1.9K
01:02Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids
3.1K
Carboxylic acids, upon heating, undergo a decarboxylation reaction by releasing carbon dioxide gas. Monocarboxylic acids do not undergo decarboxylation easily. However, a silver salt of carboxylic acid reacts with bromine or iodine under high temperature to release carbon dioxide gas and forms halide with one less carbon. This reaction is called the Hunsdiecker reaction.
3.1K
01:22Reactions of Aldehydes and Ketones: Baeyer–Villiger Oxidation
3.8K
Baeyer–Villiger oxidation converts aldehydes to carboxylic acids and ketones to esters. The reaction uses peroxy acids or peracids and is often catalyzed by acid. The reaction is named after its pioneers, Adolf von Baeyer and Victor Villiger. The reaction is achieved by a wide range of peracids such as m-chloroperoxybenzoic acid (mCPBA), perbenzoic acid (C6H5COOOH), peracetic acid (CH3COOOH), hydrogen peroxide (H2O2), and tert-butyl hydroperoxide (t-BuOOH).
The carbonyl center is...
The carbonyl center is...
3.8K
01:07Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
3.2K
Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an...
3.2K