通过抑制STING/NF-κB通路而提取的Pleione bulbocodioides的抗炎性质
Jinlei Yun1, Yindi Zhang1, Hongyan Zhang1
1Yunnan Characteristic Plant Extraction Laboratory Co., Ltd., Yunnan 650106, China.
普莱昂球化物提取物通过抑制STING/NF-κB通路显示出显著的抗炎作用. 这种兰花提取物有可能用于皮肤治疗和护肤成分.
科学领域:
- 药理学 药理学是指药理学的学科.
- 皮肤病学 皮肤病学
- 兰花科研究研究.
背景情况:
- 普莱奥内物种被珍视为装饰和药用.
- 在CITES附录II物种中,Pleione bulbocodioides具有传统的皮肤学应用,但缺乏药理学研究.
- 研究P. bulbocodioides提取物的抗炎潜力至关重要.
研究的目的:
- 为了研究P. bulbocodioides提取物 (PE) 的体外抗炎作用.
- 确定PE抗炎作用的关键生物活性化合物和分子机制.
- 评估皮肤病学应用的相关细胞和组织模型中PE的疗效.
主要方法:
- 在体外测试中,使用了用TNF-α/IFN-γ刺激的HaCaT细胞和正常人体表皮角质细胞 (NHEKs).
- 转录基因分析和西式涂抹以阐明分子标.
- 植物化学表征以确定活性成分.
- 在体内测试使用LPS诱导的RAW 264.7巨细胞和SLS刺激的3D重建的人类表皮.
主要成果:
- PE显著抑制了关键炎症基因 (IL-6,IL-1β,CCL5,CCL8,CXCL8,CXCL3和TMEM173/STING) 的表达.
- 转录组和西部斑点分析证实了PE对STING/NF-κB信号通路的抑制作用.
- 确定了米利塔林和巴塔塔辛III作为主要的生物活性化合物,负责观察到的效应.
- 在巨细胞和重建的表皮模型中,PE显示出显著的抗炎功效.
结论:
- P. bulbocodioides 提取物在体外具有强大的抗炎性质.
- STING/NF-κB信号通路是PE抗炎作用的关键目标.
- 米利塔林和巴塔塔辛III是调解这些效应的主要成分.
- 作为一种天然治疗剂或皮肤护理成分来治疗皮肤病,PE显得有前途.
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