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纤维素/基阳极的弹性变形,用于再生老化的混合培养电活性生物膜
Xue Liu1, Zheng Zhang1, Qingwen Zheng1
1Key Laboratory of Bio-based Material Science & Technology, Ministry of Education, College of Material Science and Engineering, Northeast Forestry University, Harbin, 150001, China.
概括
在生物电化学系统 (BES) 中,通过阳极材料的弹性变形,现在可以使老化的电活性生物膜 (EAB) 复原. 这种非侵入性方法提高了代谢活性和功率密度,延长了BES的运行寿命.
科学领域:
- 生物电化学系统 生物电化学系统
- 微生物的电化学
- 生物膜工程是生物膜的工程.
背景情况:
- 电活性生物膜 (EAB) 对生物电化学系统 (BES) 至关重要,但随着时间的推移,它们会降解,降低性能.
- 衰老的EAB遭受了代谢活动的减少,死细胞的积累,以及非外电原体的抑制.
- 目前用于EAB维护的方法通常是侵入性的或对长期运行无效的.
研究的目的:
- 开发和验证使用阳极材料弹性变形来复苏老年EAB的非侵入性策略.
- 研究阳极变形对生物膜结构,代谢活动和微生物群体组成的影响.
- 评估生物膜再生对BESs整体性能的影响.
主要方法:
- 合成具有高可压缩性和耐疲劳性的木质tracheid类阳极材料.
- 应用弹性变形到阳极材料在潮湿状态与老年EABs.
- 对生物膜代谢活性,细胞活力 (多SIM成像) 和细胞外聚合物质 (EPS) 组成的分析.
- 使用16S rRNA基因测序进行微生物社区分析.
- 测量BES功率密度,离子扩散,特定电容和催化电流.
主要成果:
- 阳极的弹性变形增加了37.5%的老年EAB代谢活动,并删除了死细胞.
- 观察到松结合的EPS显著减少,而EPS并不直接对电子转移作出贡献.
- 复苏抑制了非外电源的生态竞争,改善了外电源社区.
- 在功率密度上实现了显著的25.97%的增加,与离子扩散,特定电容和催化电流的改进一起.
结论:
- 弹性阳极变形是一种新且有效的非侵入性策略,用于在BES中复原老化的电活性生物膜.
- 这种机械方法通过去除死生物质和抑制性EPS,并通过促进有利的微生物群落来恢复生物膜功能.
- 这些发现对于BES的长期实际应用至关重要,并为阳极材料对微生物群落的机械影响提供了新的见解.
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