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复杂组织背景的全在一次的空间蛋白质组概况,通过近距离蛋白质组学的单细胞类型分辨率

Yiheng Mao¹, Yuan Li¹, Zhendong Zheng¹

¹Department of Chemistry and Research Center for Chemical Biology and Omics Analysis, College of Science, Guangming Advanced Research Institute, Southern University of Science and Technology, Shenzhen 518055, China.

|May 9, 2025

概括

用于绘制组织架构的空间蛋白质组学是通过空间蛋白质组学 (PSPro) 的近距离标记来推进的. 这种方法从单个组织切片中捕获具有亚微米分辨率的细胞类型蛋白质,克服了现有技术的局限性.

科学领域:

蛋白质组学是指蛋白质组学
空间生物学空间生物学
生物技术是生物技术.

背景情况:

空间蛋白质组学方法,如激光微解剖质谱法 (LMD-MS) 和抗体成像,在采样精度,吞吐量和蛋白质组覆盖率方面存在局限性.
现有的策略难以平衡详细的组织架构映射与全面的蛋白质组分析.

研究的目的:

引入空间蛋白质组学 (PSPro) 的近距离标记,作为一种用于高分辨率细胞类型蛋白质组捕获的新方法.
克服与当前空间蛋白质组学技术相关的权衡.

主要方法:

PSPro将针对抗体的生物化与亲和力净化相结合,用于从单个组织切片同时捕获蛋白质组.
该方法实现了亚微米分辨率,并与流细胞计和基于LMD的工作流进行了基准测试.
激光微解剖被集成到PSPro中,以便对同一组织内的细胞亚群进行比较分析.

主要成果:

在基准测试研究中,PSPro表现可靠.
数以千计的蛋白质,包括已知的细胞标记物,从瘤和脏组织中的十种不同的细胞类型中得到丰富.
在胰腺瘤,特别是癌症和免疫细胞中的空间蛋白质异质性通过LMD-PSPro综合分析揭示出来.

结论:

PSPro提供了一种用户友好的空间生物学方法,从"抗体-表皮质"转向"抗体-细胞类型蛋白质体"范式.
该方法增强了绘制组织结构和理解复杂生物系统中细胞类型特定蛋白质组的能力.
PSPro为研究空间蛋白质组异质性及其对癌症等疾病的影响提供了一个强大的工具.

关键词:

亲和力净化-质谱-质谱学激光微切割是微切割的方法之一.接近性标签的标签.空间蛋白质组学空间蛋白质组学组织切片组织切片

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