工程油性酵母Rhodotorula toruloides用于生产基和基
1Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
研究人员设计了酵母来生产先进的生物燃料,如和. 在Rhodotorula toruloides中的FAP途径实现了从葡萄糖和基纤维素原料中创纪录的产量.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 化石燃料的限制和碳排放推动了对可持续生物燃料的需求.
- 先进的生物燃料,如基和基,提供高能量密度和作为化石燃料替代品的潜力.
研究的目的:
- 在Rhodotorula toruloides中设计异质生物合成途径,用于和的生产.
- 评估和优化不同的途径 (AAR/ADO,Unda/UndB,FAP) 和代谢策略,以提高碳化合物产量.
主要方法:
- 三种不同的异质生物合成途径 (AAR/ADO,Unda/UndB,FAP) 的构建和比较.
- 从不同的生物体中选各种酶,以在酵母宿主中获得最佳的性能.
- 应用代谢工程策略,包括消除副产品和增强前体供应.
主要成果:
- 在AAR/ADO和UndA/UndB路径中,FAP路径表现出优越的性能.
- 从葡萄糖中获得的基和基含量为1.73 g/L,从基纤维素化物中获得的度为0.94 g/L.
- 在酵母中的和生产中建立了创纪录的高产量.
结论:
- 罗多托鲁拉 (Rhodotorula toruloides) 是碳化合物生产的可行且有前途的宿主.
- 工程化途径和优化菌株促进了从可持续原料中工业规模生产生物燃料的潜力.
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