从2000年到2023年,mcr阳性Klebsiella pneumoniae的遗传框架和进化动态
Xi-Wei Zhang1, Xi-Yi Huang2, Zhuo-Yang Zhou3
1Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), Foshan, China.
International journal of antimicrobial agents
|May 9, 2025
概括
在Klebsiella pneumoniae中,素抗性基因mcr显示出多种变异,并与碳酶基因共同发生,这表明克隆传播和需要持续监测.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 肠道细菌,特别是Klebsiella pneumoniae中胆固醇抗性基因 (mcr) 的全球传播构成了重大的公共卫生挑战.
- 了解mcr阳性K. pneumoniae的种群结构和进化史对于有效的控制策略至关重要.
研究的目的:
- 通过基因组分析,研究mcr阳性Klebsiella pneumoniae的种群结构和进化史.
- 确定mcr基因与其他耐药性和毒性因子的流行率和共发生率.
主要方法:
- 从公共数据库 (2000-2023) 中获得的mcr基因的463个Klebsiella pneumoniae序列的基因组分析.
- 检测和表征mcr变体,碳烯酶基因和超病毒性基因.
- 对mcr基因与特定基因组元素的相关性分析和人口结构分析.
主要成果:
- 检测到6种mcr变异,其中mcr-9,mcr-1和mcr-8是最常见的. 超过43%的隔离物携带了碳烯酶基因,15%的隔离物携带了超病毒性基因.
- 在特定的mcr基因 (mcr-1, -8, -9) 和基因组元素如iucABC,iutA,qxB和dfrA19.9之间发现了正相关性.
- K. pneumoniae 种群被分为 6 个群; mcr 和 carbapenemase 基因的同时出现表明跨国克隆传播. 在mcr-1和mcr-8之前,mcr-9出现了.
结论:
- mcr-1,-8和-9基因在特定地区演变为高风险血统,通常与碳烯酶或高毒性基因同时发生.
- 对mcr阳性K. pneumoniae的持续监测,特别是那些具有iucA或carbapenemase基因的人,对于公共卫生至关重要.
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