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Myd88的功能是作为一个负调节器的IRF11介导的IFN生产在远程节目中

Chao Xiang¹, Wenxing Li¹, Xiaoling Zhou¹

¹Fisheries College, Jimei University, Xiamen, 361021, China.

Developmental and comparative immunology

|May 9, 2025

概括

骨髓分化因子88 (MyD88) 对鱼类的干扰素 (IFN) 生产产生负面调节. MyD88与核中的IRF11相互作用,抑制抗病毒反应.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
鱼类生物学鱼类生物学

背景情况:

骨髓分化因子88 (MyD88) 是先天性免疫的一个关键适应蛋白,它介导来自Toll类受体 (TLRs) 和IL-1/IL-18受体的信号.
通过与IFN调节因子 (IRF) 的相互作用,MyD88在调节I型干扰素 (IFN) 信号传输中的作用已在teleosts.

研究的目的:

调查MyD88在调节IFN产生的机制性作用,由IRF11作为鱼类特定的阳性调节器进行调节.
为了阐明MyD88和IRF11之间的相互作用,在IFN信号的背景下,在teleosts.

主要方法:

在斑马鱼肝细胞中,MyD88的过度表达和敲除.
同免疫沉测试以确定MyD88和IRF11的相互作用域.
同焦点显微镜和亚细胞分离以确定亚细胞局部.

主要成果:

MyD88过度表达抑制了IRF11诱导的IFN促进体激活.
MyD88敲击增强了IFN和IFN刺激基因 (ISG) 的IRF11诱导的表达.
MyD88通过其Toll/IL-1受体 (TIR) 域和IRF11的特定区域 (aa 114-155) 与IRF11相互作用,并在核中同定位.

结论:

MyD88通过直接的蛋白质-蛋白质相互作用和核共定位来负面调节IRF11介导的IFN产生.
这项研究确定MyD88是IRF11介导的IFN生产途径中的负调节者.
提出了一种新的机制来调节涉及MyD88和IRF11的鱼类抗病毒反应.

关键词:

吉拉日本岛 (Anguilla japonica) 是一个日本岛.国际货币基金组织 IFN IFN 在 IRF11 里,你会看到 IRF11 里面.我的D8888是什么意思负面监管是一种负面监管.

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