在活细胞中液体-液体相分离过程中,FUS相互作用组的动态的时空解密
Sunfengda Song1,2, Haiyang Xie1,2, Qingwen Wang1,2
1Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Nature communications
|May 9, 2025
概括
本研究介绍了通过近距离标记辅助质谱法 (CLAPM) 组合LLPS蛋白质组的组成,这是一种新方法来跟踪活细胞内的液态相分离 (LLPS) 中的蛋白质变化. 在LLPS形成过程中,CLAPM揭示了动态蛋白质组转移,并确定了涉及的新型蛋白质.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 无膜有机体通过液态-液态相分离 (LLPS) 形成,这对细胞功能至关重要.
- 在活细胞中理解LLPS中的动态蛋白质组是具有挑战性的.
研究的目的:
- 开发一种在活细胞中LLPS过程中对蛋白质组进行时空分析的方法.
- 在不同细胞类型中识别参与LLPS的蛋白质.
主要方法:
- 通过近距离标记辅助质谱法 (CLAPM) 开发了LLPS蛋白质组合的组成.
- 使用CLAPM标记和监测细胞内滴滴中的蛋白质相互作用转移.
- 确定了来自HeLa,HEK 293T和神经细胞的LLPS滴中的蛋白质.
主要成果:
- 在LLPS滴中,CLAPM确定了129种 (HeLa),182种 (HEK 293T) 和822种 (神经元) 特定蛋白.
- 在神经细胞中,发现了596个LLPS原生蛋白,226个LLPS依赖蛋白和58个LLPS敏感蛋白.
- 在体内发现了11种以前未知的LLPS蛋白质.
结论:
- CLAPM是研究LLPS中的蛋白质的多功能工具.
- 该方法可以在LLPS期间准确地表征活细胞内的动态蛋白质组.
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