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未来的多中心研究确定预后生物标志物和微生物资料在严重的CAP使用BALF,血液mNGS和PBMC转录组学

Wanmei Song^1,2,3, Qingyuan Yang^1,2,3, Hui Lv⁴

¹Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Scientific reports

|May 9, 2025

概括

这项研究确定了严重社区获得性肺炎 (SCAP) 预后的新生物标志物. 支气管洗液的元基因组测序和转录组学揭示了与SCAP严重程度和患者结果相关的微生物和宿主遗传指标.

科学领域:

传染性疾病传染性疾病
基因组学就是基因组学.
生物标志物发现发现

背景情况:

严重的社区性肺炎 (SCAP) 是一个严重的健康挑战,预后可变.
识别可靠的生物标志物对于预测SCAP严重程度和患者结果至关重要.

研究的目的:

为了确定SCAP的潜在预后和严重性生物标志物.
为了比较支气管洗液 (BALF) 和血液下一代代基因组测序 (mNGS) 的诊断效用.
为了研究SCAP患者的转录基因变化.

主要方法:

在14名轻度CAP和75名SCAP患者的多中心前性研究中.
支气管洗液 (BALF) 和血液下一代代基因组测序 (mNGS).
周围血液单核细胞 (PBMC) 的转录和生物信息学分析 (GSEA,GO/KEGG).

主要成果:

与血液mNGS相比,BALF mNGS显示出更高的微生物多样性和病原体检测能力 (例如,Acinetobacter baumannii,Klebsigma pneumoniae,Candida albicans).
微生物标记物 (Pneumocystis jirovecii,人类细胞巨乳病毒) 和转录基因生物标记物 (OTOF,MS4A4A,SIGLEC1) 与SCAP结果不佳有关.
在已故的SCAP患者中观察到显著的免疫和代谢途径变化 (例如补体激活,氧化酸化).

结论:

在SCAP中,BALF mNGS在病原体识别方面优于血液 mNGS.
微生物和宿主转录组形状可以区分轻度和严重的CAP.
已识别的生物标志物为改善SCAP预后和治疗策略提供了潜力.

关键词:

生物标志物生物标志物预测预测预测预测这就是SCAPAP SCAP.严重程度严重程度文字转录学 (Transcriptomics) 是一个学科.mNGSS 在线观看

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