在体积干燥血斑中对内分泌蛋白质的多重量化量化
William Stauch1, Johan Olausson2,3,4, Annika Bendes1
1Science for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
定量干血斑点 (qDBS) 测试可以准确地测量从小血样中的内分泌激素. 这种微创方法在精准医学和临床诊断方面表现有前途.
科学领域:
- 生物化学 生物化学
- 内分泌学 在内分泌学.
- 临床诊断 临床诊断 临床诊断
背景情况:
- 循环中的蛋白质,包括内分泌激素,是健康和疾病的重要生物标志物.
- 传统分析依赖于静脉血液抽取的血/血清.
- 通过干血斑点 (qDBS) 进行定量毛细血管采样提供了一个最小侵入性的替代方案.
研究的目的:
- 评估量化干血斑点 (qDBS) 的分析能力,用于内分泌激素量化.
- 与血相比,使用qDBS评估多重复合免疫试验的性能.
- 确定qDBS在临床试验和精准医学中的有用性.
主要方法:
- 量化素激素子单元β (LHB),卵泡刺激激素子单元β (FSHB),甲状腺刺激激素子单元β (TSHB),益生素 (PRL) 和生长激素1 (GH1) 使用多重免疫测定在qDBS.
- 将qDBS测定结果与来自100名捐赠者的配对EDTA血样本进行了比较.
- 与经认可的临床化学实验室对比验证的蛋白质度.
主要成果:
- 多重复合免疫试验在qDBS中证明了精确的蛋白质定量 (平均CV = 8.3%) 与血的高一致性 (r = 0.880.99).
- 精度依赖于矩阵和蛋白质,血度通常高于qDBS化物 (1.27.5x).
- 在所有样本类型和测定中,在女性中观察到FSHB和LHB水平的与年龄相关的增加.
结论:
- 现代qDBS设备适用于在多重测试中量化临床信息蛋白质.
- 需要进一步的研究来优化和标准化样本的具体情况.
- 卷度DBS采样为精准医学中精确的蛋白质量定提供了新的途径.
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