相关实验视频

Updated: May 13, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

Circ_RPPH1通过招募并与EIF4 A3结合,促进膀皮质癌的扩散和EMT

HuaWei Liu¹, JunMin Ma², Xia Yan³

¹Department of Urology, Deyang People's Hospital, Sichuan Province, Deyang City, 618000, China.

|May 9, 2025

概括

循环RNA RPPH1 (circ_RPPH1) 通过干扰蛋白相互作用来促进膀癌的生长和扩散. 这项研究澄清了它在膀泌尿道癌 (BUC) 进展中的作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

循环RNA RPPH1 (circ_RPPH1) 在膀尿癌 (BUC) 中的作用尚不清楚.
驱动涉及circ_RPPH1的BUC进展的特定分子机制需要阐明.

研究的目的:

为了研究circ_RPPH1在膀泌尿腺癌 (BUC) 的参与和机制.
为了确定circ_RPPH1表达和BUC进展之间的关系.

主要方法:

定量实时PCR (RT-qPCR) 用于测量BUC组织和细胞中的circ_RPPH1水平.
功能测试包括殖民地形成,CCK-8,伤口愈合和Transwell测试,以评估扩散,迁移和入侵.
RNA免疫沉 (RIP) 和RNA拉下测试以确认分子相互作用;免疫阻塞以分析蛋白质表达.

主要成果:

在BUC组织中circ_RPPH1水平升高与瘤入侵深度增加相关.
Knockdown 的 circ_RPPH1 显著抑制了 BUC 细胞中的增殖,侵入和上皮层-介质细胞过渡 (EMT).
通过减少EIF4A3与N-cadherin和VimentinmRNA的结合,circ_RPPH1的过度表达促进了EMT.

结论:

在BUC中,Circ_RPPH1促进瘤生长和EMT.
它通过抑制EIF4A3介导的mRNA调节来起作用,从而激活EIF4A3 / N-cadherin / Vimentin通路.

关键词:

膀癌是一种癌症.电路_RPPH1 的一个字符.欧洲投资基金4 A3 A3 在EMT中,EMT是EMT.

更多相关视频

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

相关概念视频

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...