结合NF-Y与小鼠和人类细胞中的可转移元素
Mirko Ronzio1, Andrea Bernardini1, Alberto Gallo1
1Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Italy.
Mobile DNA
|May 9, 2025
概括
转录因子NF-Y与人类和小鼠基因组中的特定可转移元素 (TE) 结合. 这些相互作用表明NF-Y在通过重复的DNA序列调节基因表达方面发挥了复杂的作用.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 可移植元素 (TE) 占哺乳动物基因组的很大一部分,并包含影响基因表达的调节序列.
- 核因子Y (NF-Y) 是一种三基转录因子,它与CCAAT盒结合,对于启动编码和非编码RNA转录至关重要.
研究的目的:
- 研究人类和小鼠细胞中可移植元素上的NF-Y转录因子的结合位置.
- 在不同类型的TE中,描述其他转录因子和表观遗传标记在NF-Y结合位点的联合结合.
主要方法:
- 对8个人类和8个小鼠细胞系的NF-Y结合部位的分析.
- 特定可转移元素家族的识别 (LTR12,MLT1,MER,RLTR10,IAPLTR) 受到NF-Y的约束.
- 在NF-Y占有TE位点检查共结合转录因子丰富和表观遗传标记 (染色质可访问性).
主要成果:
- 在每个物种中,NF-Y结合仅限于特定的逆转录病毒TEs:人类的LTR12,MLT1和MER;小鼠的RLTR10和IAPLTR.
- 不同的转录因子 (例如,MAFK/F/G,USF1/2,TALE TFs) 在不同TE类的NF-Y结合部位附近被丰富.
- NF-Y与MER元素中的开放色素,LTR12中的闭合色素和MLT1中的混合状态相关,许多位点作为增强剂产生eRNAs.
结论:
- 在哺乳动物基因组中,NF-Y对不同类别的可移植元素表现出特定的结合偏好.
- 与其他转录因子和表观遗传状态的共同关联表明NF-Y在重复序列上具有复杂的调节作用.
- 这些发现突出了转录因子,TE和基因调节在塑造哺乳动物基因组之间的复杂相互作用.
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