在禁食和非禁食状态下急性相反应蛋白的波动和变化
Ben Huang1,2, Shuxian Miao1,2, Yan Xu1,2
1Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Journal of clinical laboratory analysis
|May 10, 2025
概括
禁食状态显著影响急性相反应蛋白 (APRP) 测试结果. 像CRP和IL-6这样的APRP的食后变化可能超过可接受的临床误差幅度,影响诊断.
科学领域:
- 临床化学 临床化学
- 生物标记分析 生物标记分析
- 诊断测试 诊断测试 诊断测试
背景情况:
- 在临床实践中,急性相反应蛋白 (APRP) 是常规测量的.
- 当前的协议通常涉及随机取血样,没有标准化的禁食指南.
- 不禁食与禁食状态对APRP水平的影响仍然不清楚.
研究的目的:
- 调查禁食和非禁食条件之间的APRP水平的变化.
- 评估APRP中食后波动的临床意义.
主要方法:
- 用口服葡萄糖耐受性测试 (OGTT) 来标准化能量摄入量和时间.
- 50名受试者进行了12小时的禁食,在基线 (禁食) 和在血糖后的30,60,120和180分钟内收集血液样本.
- 使用百分比偏差和布兰德-阿尔特曼图表来量化变化.
主要成果:
- 在多个APRP中观察到显著的食后变化.
- 在服用后的32-46%的受试者中,C-反应蛋白 (CRP) 显示出临床上不可接受的偏差.
- 在54%-64%的受试者中,介质素-6 (IL-6) 呈现出不可接受的波动,其他APRP也显示出显著的变化.
结论:
- 饮食摄入导致APRP水平的显著临床波动.
- 临床医生必须考虑最近的食物消费对APRP测试结果的影响.
- 标准化的预分析条件,包括禁食,对于准确的APRP解释至关重要.
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