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Szarina Krisha K Ko¹, Rohani Cena-Navarro², Ma Jowina H Galarion¹

¹National Institute of Molecular Biology and Biotechnology, National Institutes of Health, University of the Philippines Manila, 1000, Manila, Philippines.

Archives of virology

|May 10, 2025

概括

非洲猪瘟 (ASF) 正在影响菲律宾. 对非洲猪瘟病毒 (ASFV) 的基因组分析揭示了其基因型和在该国传播的潜在变种.

科学领域:

兽医病毒学兽医病毒学
基因组学就是基因组学.
流行病学流行病学

背景情况:

非洲猪瘟 (ASF) 是一种高度传染性的病毒性疾病,影响家养猪和野猪.
最近在亚洲,欧洲和加勒比地区爆发的疫情,影响了菲律宾90%的省份,突显了急需进行菌株表征的迫切需要.
对于目前在菲律宾传播的非洲猪瘟病毒 (ASFV) 存在有限的基因组信息.

研究的目的:

为菲律宾最近爆发的ASFV菌株提供全面的基因组概况.
确定流通中的ASFV的基因型和亚基因型.
与现有的参考菌株相比,识别潜在的病毒变异.

主要方法:

在没有先前放大的情况下,对2020年国内农场爆发的血液样本进行了全基因组测序.
一个定制的生物信息学管道被用于基因组组装和分析.
基因组数据被分析以确定病毒基因型,并识别遗传标记和变异.

主要成果:

成功组装了两个ASFV基因组的80-99%.
确认流通的ASFV菌株属于基因型2.
确定了特定的亚基因型概况,并检测出了与参考菌株相关的潜在变异.

结论:

这项研究提供了菲律宾ASFV的关键基线基因组数据.
了解循环菌株的分子特征对于疾病控制和预防战略至关重要.
这些发现有助于全球对ASFV流行病学和演变的理解.

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