在微生物硫化物过程中通过元素硫不成比例的反抑制来自硫化
Guijiao Zhang1, Hao-Ran Xu1, Wenwei Liao1
1State Key Laboratory of Urban Water Resources and Environment, School of Eco-Environment, Harbin Institute of Technology Shenzhen, Shenzhen 518055, PR China.
Water research
|May 10, 2025
概括
元素硫不成比例 (S0Disp) 产生硫化物,抑制了这个过程. 这项研究量化了Desulfocapsa培养中的硫化物反抑制 (FBI),确定H2S作为抑制剂并提出代谢机制.
科学领域:
- 生物地质化学生物地质化学
- 微生物生态学 微生物生态学
- 环境微生物学 环境微生物学
背景情况:
- 元素硫不成比例 (S0Disp) 是硫循环和废水处理中的一个关键的硫化过程.
- 了解硫化物反抑制 (FBI) 对于硫化物生成过程至关重要,但对于S0 Disp.它仍未得到充分研究.
研究的目的:
- 系统地研究元素硫不成比例的反抑制 (FBI).
- 为了识别抑制硫化物种并量化其抑制度.
- 为了阐明代谢途径和机制,底层的H2S衍生FBI在S0Disp.
主要方法:
- 用Desulfocapsa丰富的混合作物的种植.
- 硫化物反抑制 (FBI) 的量化和抑制度的确定 (IC50,IC值).
- 甲基转录组分析以推断代谢途径和机制.
主要成果:
- 硫化 (H2S) 被确定为S0的直接抑制剂.
- 半最大抑制度 (IC50-H2S) 是22 mg-S/L,并且值抑制度 (ICthreshold-H2S) 是40 mg-S/L.
- 在ICthreshold-H2S时,FBI从可逆转变为不可逆转,可能涉及血红酶失活和基质缺乏.
结论:
- 这项研究提供了对H2S的元素硫不成比例 (S0 Disp) 的反抑制的关键见解.
- 研究结果阐明了H2S衍生的FBI的机制,包括酶失活和基质限制.
- 结果将有助于理解自然硫循环,并优化基于S0的盘式废水处理系统.
相关概念视频
Preparation and Reactions of Sulfides
4.7K
Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
4.7K
The Sulfur Cycle
43.3K
Sulfur, an important element in the chemical makeup of proteins, is recycled through the atmosphere and aquatic and terrestrial environments. Found in the atmosphere as sulfur dioxide (SO2), sulfur is released by decaying organisms, weathered rocks, geothermal vents, volcanos, and burning fossil fuels. It is deposited into the ecosystem, cycled through the biotic community, and either released back into the atmosphere as gas or deposited in marine sediment for long-term storage and eventual...
43.3K
Electrophilic Aromatic Substitution: Sulfonation of Benzene
5.6K
Sulfonation of benzene is a reaction wherein benzene is treated with fuming sulfuric acid at room temperature to produce benzenesulfonic acid. Fuming sulfuric acid is a mixture of sulfur trioxide and concentrated sulfuric acid.
5.6K
Preparation and Reactions of Thiols
5.9K
Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.
5.9K
Feedback Inhibition
53.4K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
53.4K
Precipitation and Co-precipitation
1.6K
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
1.6K


