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在海黄瓜Apostichopus japonicus中使用转录组分析识别和描述与疾病耐药性相关的SNP

Liyuan Guo¹, Lianlian Sun¹, Chenghua Li²

¹State Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo, Zhejiang, 315211, China.

Fish & shellfish immunology

|May 10, 2025

概括

研究人员确定了与Apostichopus japonicus的抗病性相关的单核酸多态性 (SNP) 标记物. 这些标记物可以改善对抗病性海的繁殖计划,这对水产养殖至关重要.

科学领域:

水产养殖是水产养殖的一种方式.
遗传学是一个遗传学.
海洋生物学海洋生物学

背景情况:

日本 (Apostichopus japonicus) 是一种有价值的水产养殖物种,由于Vibrio splendidus引起的皮肤综合征 (SUS) 而面临重大损失.
分子育种策略,包括标记器辅助选择,对于开发抗病菌株至关重要.

研究的目的:

为了识别与Apostichopus japonicus的疾病耐药性相关的单核酸多态 (SNP) 标志物.
利用转录组数据用于标记物发现,以加强水产养殖繁殖计划.

主要方法:

对抗疾病 (RG) 和易受疾病 (SG) 的Apostichopus japonicus群体进行比较转录组分析.
单核酸多态 (SNP) 标记物的识别和关联分析.
具有显著SNP的基因的功能注释.
使用桑格测序验证候选SNP.

主要成果:

在RG和SG组之间,确定了超过560万个SNP标记.
365个基因中的503个SNP显示出显著的等位基因失衡,与免疫相关的基因在溶酶体通路中丰富.
三个经过验证的SNP证明了与疾病耐药性具有统计学意义的关联.

结论:

已识别的SNP是Apostichopus japonicus繁殖中标记辅助选择的有希望的分子标记物.
这项研究支持开发抗病性海菌株,减轻水产养殖业的经济损失.

关键词:

亚波斯蒂科普斯 (Apostichopus japonicus) 是一个日本动物.疾病耐药性疾病耐药性在SNP中,SNP是SNP.转录组转录组就是一个转录组.这种病毒叫做Vibrio splendidus.

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相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...