通过双阶段氧化刺激策略改善Pleurotus citrinopileatus中的ergothioneine含量
Weiyi Li1, Yuwan Weng1, Huiguo Ma1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Bioresource technology
|May 10, 2025
概括
研究人员通过使用一种新的两阶段氧化刺激剂,提高了中的ergothioneine (EGT) 产量. 这种方法显著提高了EGT水平,减少了发酵时间,为工业生物合成提供了途径.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 营养保健品 营养保健品
背景情况:
- 是一种有价值的农作物,含有营养素和生物活性化合物,如ergothioneine (EGT).
- 欧戈氨酸 (EGT) 是一种强大的抗氧化剂,具有显著的健康益处,使其增强的生产是可取的.
- 目前用于EGT生物合成的方法需要优化以实现工业可扩展性.
研究的目的:
- 开发一种有效的策略,以提高宏观真菌中欧戈氨酸 (EGT) 的含量.
- 为最大限度地生产EGT,优化P. citrinopileatus 303的种植条件.
- 研究一种两阶段氧化刺激策略的有效性,以促进EGT生物合成.
主要方法:
- 对11种宏观真菌物种进行选,以确定最好的EGT生产者.
- 优化P. citrinopileatus的种植条件 303.优化种植条件
- 应用一种两阶段的氧化刺激,涉及过氧化 (H2O2) 和维生素C,用于氧化清除.
主要成果:
- P. citrinopileatus 303被确定为EGT生产的最佳菌株.
- 经过优化栽培,EGT标位达到533.93 mg/L.
- 两个阶段的氧化刺激策略将EGT标位提高到641.76 mg/L (增加5.83倍),并将发酵时间缩短到5天.
结论:
- 一个两阶段的氧化刺激策略是高度有效的增强EGT生物合成在P. citrinopileatus.
- 这种优化的方法为工业规模的欧戈氨酸生产提供了一种可行的方法.
- 这些发现为改善这种重要的抗氧化化合物的生物合成提供了宝贵的见解.
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