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麦克费里丁与McNrf2合作,减轻子[a]烯诱导的氧化应激在厚贝Mytilus coruscuscus中

Jiemei Zhao¹, Tianyi Liang¹, Mingshan Song¹

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang 316004, PR China; Zhoushan Fishery Breeding and Hatching Innovation Center, Zhoushan, Zhejiang 316004, PR China.

International journal of biological macromolecules

|May 10, 2025

概括

这项研究揭示了鱼费里丁 (McFerritin) 和核因素红色素2相关因子2 (Nrf2) 共同作用,保护海洋生物免受[a]pyrene (B[a]P) 诱导的氧化应激.

科学领域:

海洋生物学海洋生物学
环境毒理学环境毒理学
分子生物学分子生物学

背景情况:

多环芳 (PAH),如 (B[a]P),通过氧化压力对海洋生物造成重大生态损害.
核因子红色素2相关因子2 (Nrf2) 调节抗氧化防御,但其与铁蛋白的相互作用在软体动物中尚不清楚.

研究的目的:

在Mytilus coruscus中识别和表征一种新的费里同类物 (McFerritin).
调查麦克费里丁在[a]皮林 (B[a]P) 诱导的氧化应激中的作用.
为了阐明McFerritin和McNrf2在Mytilus coruscus中的相互作用.

主要方法:

使用定量PCR分析不同组织中的McFerritin基因表达和B[a]P暴露后.
使用GST拉下测试来确认McFerritin和McNrf2.2之间的直接相互作用.
用RNA干扰 (RNAi) 来使McFerritin和McNrf2基因沉默,以评估它们的相互调节和对氧化应激标志物的影响.

主要成果:

作为B[a]P暴露的反应,McFerritin表达显著上调,特别是在消化腺和地幔中.
证实了McFerritin和McNrf2之间的直接相互作用.
击败麦克费里丁或McNrf2导致了亡的增加,反应性氧物种 (ROS) 水平的升高和抗氧化酶活性的降低,表明它们的协同保护作用.

结论:

费里在Mytilus coruscus中起着至关重要的保护作用,防止B[a]P诱导的氧化应激.
麦克费里丁和McNrf2之间的相互作用对于调解软体动物的抗氧化剂防御机制至关重要.
这项研究增强了对海洋无脊椎动物氧化应激调节的理解,并突出了它们作为PAH污染生物标志物的潜力.

关键词:

子[a]二烯是一种二烯.费里胺是一种铁素.这种植物是Mytilus coruscuscus.核因子红色素2相关因子2的核因子.氧化应激是一种氧化应激.

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