来自Limosilactobacillus fermentum的新型外聚糖 A10:生物合成,物理化学性质和抗氧化活性
Guangqiang Wei1, Wenbin Zhang1, Yufang Li1
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
International journal of biological macromolecules
|May 10, 2025
概括
这项研究表明,来自Limosilactobacillus fermentum A10的新型外聚糖EPS-1具有显著的抗氧化特性. EPS-1激活了Keap1-Nrf2/ARE通路,显示了功能性食物的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 在Limosilactobacillus fermentum A10中,可以产生新型的外聚糖 (EPS).
- 了解EPS生物合成和特性对于功能性食品应用至关重要.
- 微生物EPS的抗氧化机制对研究越来越感兴趣.
研究的目的:
- 研究一种来自Limosilactobacillus fermentum A10.的新型EPS的生物合成,物理化学特性和抗氧化活性.
- 阐明了孤立EPS的抗氧化作用背后的分子机制.
- 评估这种EPS在功能性食品中使用的潜力.
主要方法:
- 全基因组测序和功能注释用于基因识别.
- DEAE-52纤维素柱色谱用于EPS净化.
- 结构分析,热稳定性测试,激素清除试验和基于细胞的试验 (HepG2细胞).
- Keap1-Nrf2/ARE信号通路激活分析和分子对接.
主要成果:
- 一种新型的异多聚糖,EPS-1,由拉姆诺斯,银河糖,葡萄糖,曼诺斯和葡萄糖酸组成,被分离和表征.
- EPS-1表现出极好的热稳定性 (高达257.80°C),zeta电位为-18mV,粒子大小为146nm.
- EPS-1表现出显著的激素清除活性,对氧化损伤的细胞保护作用,以及增强的抗氧化酶活性 (SOD,GSH-Px,CAT).
- EPS-1激活了Keap1-Nrf2/ARE信号通路,分子对接揭示了EPS组件和Keap1蛋白之间的关键相互作用.
结论:
- 来自Limosilactobacillus fermentum A10的EPS-1是一种有前途的功能成分,具有强大的抗氧化能力.
- 抗氧化活性通过Keap1-Nrf2/ARE通路的激活来调节.
- EPS-1具有开发的潜力,作为一种功能性食品成分,针对氧化应激.
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