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细菌是否可以通过脂质封装稳定,当它被雾化以供吸入传递Pseudomonas aeruginosa时?

Yue Cao¹, Yuncheng Wang², Mengyu Li¹

¹Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney, NSW 2006, Australia.

International journal of pharmaceutics

|May 10, 2025

概括

脂质体封装保护菌体 (菌体) 在雾化过程中免受损伤,改善了呼吸道感染的吸入菌体治疗. 这种方法保持了菌体的活力和生物活性,以有效地向多药耐药病原体输送气溶.

科学领域:

生物技术是生物技术.
制药科学制药科学
微生物学微生物学

背景情况:

吸入的细菌体 (菌体) 疗法对治疗多药耐药 (MDR) 呼吸道病原体如Pseudomonas aeruginosa.显示出希望.
气溶输送的雾化可以损害菌体的稳定性,导致由于机械应力而导致显著的标位损失.

研究的目的:

评估脂质体封装在化过程中保护菌体的疗效.
评估脂质体封装对吸入疗法的菌体活力和生物活性的影响.

主要方法:

两种P. aeruginosa菌体 (PEV2和PEV40) 被封装在用DSPC,胆固醇,Tween 80和DOTAP配制的脂质体中.
用喷射和振动网状装置将脂质体封装和非封装的菌体雾化.
在雾化后分析了菌体活力,标位损失,粒子大小和可呼吸分数.

主要成果:

实现了高封装效率 (78%的PEV2,90%的PEV40) 的颗粒大小分别为300nm和650nm.
质体封装的菌体显示出最小的标位损失 (<0.4 log10),在雾化后保持活力.
非封装的菌体经历了大量的标位减少 (高达1.23 log10),特别是在喷气雾化中.
振动网雾化为封装的菌体产生了更好的菌体恢复 (> 90%) 和可呼吸的部分 (> 70%).

结论:

脂质封装有效地保护细菌体免受通过雾化在气溶化过程中的机械损伤.
这种保护策略维持了菌体的生物活性,提高了针对呼吸道感染的成功吸入菌体治疗的潜力.

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