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肠道微生物群驱动外部益生菌的结构变异,以增强殖民化

Shuaiming Jiang¹, Shi Huang², Zeng Zhang¹

¹School of Food Science and Engineering, Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, China.

|May 11, 2025

概括

益生菌细菌通过突变适应肠道环境,包括遗传插入,显示出更好的生存和殖民. 这种"肠道化"提供了一个基于生态的战略,以提高益生菌的有效性.

科学领域:

微生物学微生物学
基因组学就是基因组学.
益生菌益生菌是一种益生菌.

背景情况:

益生菌面临来自本地肠道微生物群的殖民抵抗,限制了它们的有效性.
益生菌的基因工程是不是普遍适用的,因为个体肠道微生物群的变化.

研究的目的:

为了研究肠道适应的益生菌*Lactiplantibacillus plantarum* HNU082 (Lp082) 的突变是否可以克服殖民抵抗.
了解推动肠道环境中益生菌适应的遗传和进化机制.

主要方法:

基于培养的方法和元基因组学被用来分析小鼠肠道中的Lp082进化.
纳米孔测序用于识别益生菌菌株中的结构变异 (SV).
现型分析评估了在胆盐压力下抗酸性和生长.

主要成果:

确定了Lp082的两个不同的进化类,其中一个类由于突变增加而表现出增强的生存能力.
虽然观察到单核酸变异 (SNV),但结构变异,特别是Cro/C1型HTHDNA结合域插入,至关重要.
带有这些插入的12个菌株在胆盐压力下表现出增强的生长和繁殖,导致肠道殖民增加.

结论:

肠道化过程驱使益生菌积累SNV和SV,增强它们的殖民能力.
这项研究为益生菌殖民机制提供了新的见解.
提出了一种基于生态的战略,以改善益生菌的殖民和有效性.

关键词:

适应肠道的菌株乳植物菌种植物植物 HNU08282 殖民化殖民化的殖民化不同的进化是不同的进化.益生菌是一种细菌.结构变化结构变化

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