在主流香烟烟雾中同时分析9种芳香氨基,使用修改后的QuEChERS和液体染色学-并联质谱法
Jie Zhang1, Tongguang Xu1, Xiang Chang1
1Shanghai Tobacco Group Corporation of CNTC, Beijing, China.
Journal of separation science
|May 11, 2025
概括
一种新的QuEChERS方法与LC-MS/MS有效地检测到香烟烟雾中的九种芳香胺. 这种经过验证的方法为分析烟草制品中的有害化合物提供了更好的灵敏度和准确性.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
背景情况:
- 芳香胺是常见的香烟烟雾中发现的有害化合物.
- 对这些氨基的准确量化对于公共卫生和监管目的至关重要.
- 目前在香烟烟雾中检测芳香胺的方法往往缺乏效率和灵敏度.
研究的目的:
- 开发和验证一种经过修改的QuEChERS方法与LC-MS/MS相结合,用于在主流香烟烟雾中同时检测九种芳香胺.
- 提高收集效率,减少烟雾样本分析中的矩阵效应.
- 通过合作研究来评估该方法的性能,并将其应用于商业香烟样本.
主要方法:
- 采用了经过修改的QuEChERS (快速,轻松,便宜,坚固和安全) 方法.
- 使用高性能液体染色学-双重质谱法 (LC-MS/MS) 进行检测和量化.
- 使用初级二次胺和C18的专用过片和净化步骤被用于增强样本清洁和减少矩阵效应.
主要成果:
- 开发的方法证明了芳香胺的高收集效率.
- 通过低检测极限 (0.02-0.10 ng/cig),高回收率 (86.1%-113.7%) 和良好的精度 (日内和日间<14%) 实现了卓越的分析性能.
- 参与10个实验室的合作研究验证了该方法的稳定性,平均可重复性和可重复性相对标准偏差在可接受范围内.
结论:
- 修改后的QuEChERS-LC-MS/MS方法提供了一种灵敏,高效和准确的方法,用于确定主流香烟烟雾中的九种芳香胺.
- 这种经过验证的方法比现有技术具有优势,包括提高精度和降低成本.
- 该方法在中国商用香烟上的应用证明了其在监管和研究目的中的实际实用性.
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