模块SlDOF9-SlSWEET17:用于控制茄子中尼马诱导的胆和根之间的糖分布的开关
Xiaoyun Wang1,2, Zhimei Wang1,2, Xinyue Tang1,2
1College of Plant Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China.
概括
茄子 番茄子 番茄子
科学领域:
- 植物病原体相互作用
- 分子植物病理学 分子植物病理学
- 植物生理学 植物生理学
背景情况:
- 营养物质的分配对于植物健康和抗病能力至关重要.
- 根结线虫 (RKN) 是消耗植物糖的植物病原体.
- 甜蛋白 (糖最终将被出口运输体) 是植物中糖运输的关键调节者.
研究的目的:
- 研究Slsweet17在番茄对RKN感染的反应中的作用.
- 确定参与RKN易受性的SlSWEET17的上游调节器.
主要方法:
- SlSWEET17的亚细胞定位.
- 对SLSWEET17蛋白相互作用的分析.
- 在番茄中进行基因表达和淘汰突变研究.
- 染色体免疫沉 (ChIP) 试验.
- 电泳运动移动性转换试验 (EMSA).
- 双露西法酶记者测定.
主要成果:
- SlSWEET17局限于血,戈尔吉和真空孔,并且在RKN诱导的胆汁中高度表达.
- 通过与其他SLSWEET蛋白相互作用,SLSWEET17通过与其他SLSWEET蛋白相互作用来负面调节糖运输.
- 过度表达SlSWEET17会降低胆汁糖含量和RKN易感性;淘汰突变体显示易感性增加.
- SlDOF9 作为 SlSWEET17 的上游负调节器.
- 过度表达SlDOF9会增加胆汁糖含量和RKN易感性;淘汰突变者显示易感性降低.
结论:
- 该SlDOF9-SlSWEET17调节模块控制糖分成RKN胆,影响植物的易感性.
- 准这种途径为管理番茄中RKN疾病提供了潜在的策略.
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