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莱迪格和塞尔托利细胞对双A的敏感性差异

Do-Yeal Ryu¹, Won-Ki Pang², Md Saidur Rahman²

¹Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea; Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

|May 11, 2025

概括

双甲 (BPA) 暴露会通过损害莱迪格和塞尔托利细胞影响男性生育能力. 不同的BPA度显示出不同的细胞损伤机制和敏感性,影响的合成和细胞完整性.

科学领域:

内分泌学在内分泌学.
毒理学毒理学毒理学
生殖生物学生殖生物学

背景情况:

双A (BPA) 是一种无处不在的内分泌干扰化学物质,具有已知的毒性.
通过对莱迪格和塞尔托利细胞的不良影响,BPA暴露与男性不孕症有关.
在生殖细胞中BPA的机制和特定的有毒度需要进一步阐明.

研究的目的:

研究BPA对莱迪格和塞尔托利细胞的剂量依赖作用和潜在机制.
为了确定病态度和细胞对不同BPA暴露的反应.
为了比较每个细胞类型中不同的敏感性和受影响的分子通路.

主要方法:

用BPA (0.0001100μM) 的连续稀释处理莱迪格和塞尔托利细胞.
评估线粒体功能 (膜潜力,ATP水平),反应性氧物种 (ROS) 生产和亡.
对类固醇酶和结合蛋白的mRNA表达的分析,以及雌激素受体α和蛋白酶A信号传递.

主要成果:

低BPA度降低了线粒体膜潜力和ATP;高度增加了两种细胞类型的ROS水平.
观察到线粒体介导的亡,莱迪格细胞和塞尔托利细胞之间的敏感性差异.
通过雌激素受体α的介导,BPA对莱迪格细胞中的类固醇生成和合成产生了负面影响,并减少了塞尔托利细胞中的结点蛋白.

结论:

BPA对莱迪格和塞尔托利细胞产生明显的毒性作用,影响男性生殖健康.
细胞对BPA的反应涉及改变的线粒体动力学,亡和特定的分子信号通路.
雌激素受体α介导BPA效应,而蛋白激酶A显示相反的表达模式,突出细胞特异性敏感性.

关键词:

双甲 (BPA) 是一种莱迪格细胞是莱迪格细胞.男人生育能力男人生育能力线粒体中的线粒体.塞尔托利细胞是Sertoli细胞.

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