有证据表明,乳牛的保留胎盘中,细胞巨细胞的偏振不平衡

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Updated: May 13, 2025

Isolation of Leukocytes from the Human Maternal-fetal Interface

Isolation of Leukocytes from the Human Maternal-fetal Interface

Published on: May 21, 2015

Huijing Zhang¹, Ming Li¹, Bichen Zhao¹

¹College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China.

Journal of dairy science

|May 11, 2025

概括

乳牛中保留的胎盘与较少的细胞巨有关. 这些巨细胞表现出M2主导的两极分化,通过减少细胞亡和组织重塑,损害了胎盘驱逐.

科学领域:

兽医医学兽医医学兽医医学
生殖生物学生殖生物学
免疫学免疫学免疫学

背景情况:

保留的胎盘 (RP) 影响8%的奶牛,原因复杂.
巨细胞对于胎盘驱逐至关重要,调解组织重塑和免疫反应.

研究的目的:

调查RP和没有RP的母胎界面上的形态变化.
量化,定位,并确定RP奶牛中细胞巨细胞的两极分化.
在RP中探索巨细胞变化和胎盘病理之间的联系.

主要方法:

从80头荷尔斯坦乳牛收集了血液和胎盘组织.
根据胎盘驱逐时间将奶牛分为正常驱逐 (NE) 和RP组.
通过mRNA和蛋白质表达和免疫光学分析了原体沉积,矩阵金属蛋白酶,亡标记物,化疗因子和巨细胞标记物 (CD68,CD206,CD86).

主要成果:

RP奶牛的原III和TIMP-1的增加,MMP-2和MMP-9的减少.
在RP奶牛中观察到降低的亡 (较低的BAX,Caspase-3/8;较高的BCL2) 和改变的免疫因子 (较低的CXCL1/MCP-1,IL-1β;较高的IL-10).
RP奶牛的CD68+巨细胞较少,转向M2极化 (较高的CD206,IL-10,IL-6,TGF-β) 和减少的M1标记物 (CD86,iNOS,IL-1β,NF-κB).

结论:

乳母牛保留胎盘的乳母牛表现出一个减少的丸巨细胞群.
巨细胞极化转向RP中占主导地位的M2表型,可能阻碍胎盘驱逐.
改变母胎界面的巨细胞数量和表型可能会损害胎盘释放的必不可少的亡和蛋白质分解过程.

关键词:

在M2巨细胞中.卡龙克巨巨巨乳牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛保留了胎盘的胎盘.

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Transcytosis of IgG

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