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在大肠杆菌表达的Bacillus thuringiensis Cry1Ia36的杀菌活性

Feixue Cheng¹, Dongwei Wang¹, Jian Wang²

¹Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province, Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.

Pesticide biochemistry and physiology

|May 11, 2025

概括

甲状腺菌 (Bt) Cry1Ia36蛋白对植物寄生性线虫 (PPNs) 具有强大的杀菌活性. 这一发现为农作物保护提供了一个新的生物控制策略,减少了对化学农药的依赖.

科学领域:

农业科学农业科学
生物技术是生物技术.
分子生物学分子生物学

背景情况:

植物寄生性线虫 (PPNs) 对全球农业构成重大威胁.
生物控制剂,如Bacillus thuringiensis (Bt) 和其晶体蛋白 (ICPs),为化学农药提供了一个可持续的替代品.
针对PPN的特定Bt ICPs的杀菌潜力在很大程度上仍未被探索.

研究的目的:

为了研究Cry1Ia36蛋白质从Bacillus thuringiensis对植物寄生虫线虫Meloidogyne incognita的杀菌活性.
评估Cry1Ia36在控制植物中M.隐形菌感染方面的疗效.
为了阐明Cry1Ia36杀菌作用背后的分子机制.

主要方法:

在大肠杆菌中表达了cry1Ia36基因,并净化了产生的蛋白质.
在M. incognita J2s.上使用LC50测定来评估Nematicidal活性.
实验评估了对黄瓜根的控制效应,并产生表达cry1Ia36的转基因番茄.
转录组分析和酵母二杂交试验被用于识别相互作用蛋白并了解作用模式.

主要成果:

纯化的Cry1Ia36蛋白对M. incognita J2s表现出显著的杀菌活性,96小时的LC50为12.79 mg/L.
Cry1Ia36在黄瓜中对M. incognita根部感染产生了76.95%的控制效果.
表达cry1Ia36的转基因番茄显示胆汁和卵质形成减少.
转录组分析揭示了受治疗的线虫中的显著基因表达变化,并确定了一种相互作用的蛋白质.

结论:

Cry1Ia36蛋白是一种强大的杀虫剂,可以对抗Meloidogyne incognita.
Cry1Ia36 是一个有前途的生物控制候选人,用于农业中管理PNP疾病.
这项研究扩大了Bacillus thuringiensis晶体蛋白的已知应用范围,并提供了一个环保的替代品.

关键词:

细菌 thuringiensis 这种疾病.在Cry1Ia36蛋白质中,Cry1Ia36蛋白质是最重要的.相互作用蛋白质的相互作用梅洛伊多基尼隐形的身份.类杀虫活动类杀虫活动植物寄生性线虫虫是一种植物寄生性线虫.

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