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在模拟微生物和光合作用平台中,通过异质基合成的工程无氧电子流通过异质基合成

Haruki Sasaki¹, Yuri Yanagihara¹, Yui Satofuka¹

¹Department of Applied Biological Chemistry, Faculty of Agriculture, Osaka Metropolitan University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.

Journal of bioscience and bioengineering

|May 11, 2025

概括

罗多 (RQ) 合成通过改善氧化还原平衡和电子运输来增强无氧生物生产. 这种代谢工程工具在氧气有限的条件下,在细菌和真核生物系统中提高了酸盐产量.

科学领域:

代谢工程是代谢工程.
生物技术是生物技术.
生物化学生物化学

背景情况:

无氧生物生产受到破坏的氧化还原平衡和低效的电子传输的限制.
罗多 (RQ) 是一种具有低氧化还原潜力的,可以支持无氧电子运输.
从ubiquinone通过rhodoquinone生物合成蛋白A (RquA) 来合成RQ是一种简单的代谢工程策略.

研究的目的:

使用RQ合成设计无氧代谢途径.
在氧气有限的条件下提高生物生产效率.
为了证明RQ合成在不同生物系统中的多功能性.

主要方法:

在Escherichia coli中Euglena gracilis rquA的异质表达 ΔmenA.
在Cyanidioschyzon merolae线粒体中表达Rhodospirillum rubrum rquA的表达.
分析糖酸盐生产,氧化还原因子比率和电子运输.

主要成果:

改造后的大肠杆菌增加了三倍的酸盐产量,并显示出更好的电子流量 (降低了NADH/NAD+比率).
经过工程设计的C. merolae在无氧状态下产生了更多的苏酸盐,并改变了线粒体电子运输.
证明RQ合成与现有的代谢修饰相兼容,并且在细菌和真核线粒体中具有功能.

结论:

RQ合成是一种提高无氧代谢和生物生产的多功能工具.
这种方法为在氧气有限的条件下代谢工程提供了一个简单而有效的策略.
在改善微生物和真核细胞工厂方面,RQ合成具有广泛的应用.

关键词:

无氧生产是一种无氧生产.生物炼油厂是一个生物炼油厂.二氧化碳的含量是多少? 二氧化碳的含量是多少埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.代谢工程是代谢工程.转氧化物平衡还原平衡.罗多基诺的使用方法苏酸盐是一种酸盐.

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