相关实验视频

Updated: Jun 24, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Published on: May 16, 2012

[选择和验证用于定量实时PCR分析的参考基因在Tujia医学中Xuetong]

Qian Xiao1, Chen-Si Tan1, Jiang Zeng1

  • 1School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Traditional Chinese Medicine and Ethnomedicine Innovation & Development International Laboratory,Hunan University of Chinese Medicine Changsha 410208, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
|May 12, 2025
PubMed
概括

这项研究确定了UBC作为Kadsura heteroclita分子研究的稳定参考基因. 在不同的植物部位中,UBC和RPL是稳定的,而UBC和GAPDH在各种压力条件下是稳定的,验证了其在基因表达分析中的使用.

关键词:
卡德苏拉 (Kadsura) 是一个异质聚合物.美国UBCBC公司实时PCR定量的实时PCR.基因参考基因是指基因的参考基因.

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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...