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Updated: May 14, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

在Komagataella phaffii中识别和功能分析与生长速度相关的长非编码RNA

Benjamin Luke Coltman^1,2,3, Krishna Motheramgari⁴, Nadine Tatto^2,4,5

¹CD-Laboratory for Growth-decoupled Protein Production in Yeast at Department of Biotechnology, BOKU University, Vienna, Austria.

Computational and structural biotechnology journal

|May 12, 2025

概括

研究人员在酵母 Komagataella phaffii.中鉴定出168种新的长非编码RNA (lncRNA). 这些lncRNA很可能通过调节基因表达来帮助酵母适应压力,缓慢生长的条件.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
酵母遗传学酵母遗传学

背景情况:

长非编码RNAs (lncRNAs) 是关键的调节分子,参与转录和转化层次的基因表达.
鉴定lncRNAs是具有挑战性的,基因组引导的RNA-Seq数据映射是最可靠的方法.
lncRNA表达的变化往往与酵母体的细胞应激反应有关.

研究的目的:

在接近零生长条件下识别和描述酵母 Komagataella phaffii 中的 lncRNA.
研究 lncRNAs 在酵母适应环境压力的潜在调节作用.
探索lncRNA表达与潜在交互伙伴的关联.

主要方法:

使用了K. phaffii在葡萄糖限制的静止剂中培养的RNA-Seq数据.
采用适应的生物信息管道用于lncRNA识别.
分析了与生长相关的变化和潜在的相互作用伙伴相关的lncRNA表达水平.

主要成果:

在K. phaffii.中鉴定出168种主要是新型的lncRNA.
发现36个 lncRNA 呈现与生长相关的表达变化.
观察到 lncRNA 表达与潜在的 cis 和 trans 作用伙伴之间的关联.

结论:

lncRNA在K. phaffii对环境变化,特别是缓慢生长条件的适应性反应中发挥着重要作用.
鉴定到的lncRNAs表明了新的调节机制,有助于酵母的生存和适应.
这项研究扩大了对非传统酵母菌种中lncRNA功能的理解.

关键词:

科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.接近零的增长率接近于零.这就是Pichia pastoris.保持电源的状态.在ncRNA中,我们可以

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