使用平行阵列微流体柱液体染色学 (palmLC) 选择正确的C18静止相.
Hanchen Cao1, Tianyue Feng1, Kaiming Ji2
1Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361005, China.
Analytical chemistry
|May 12, 2025
概括
一个新的平行阵列微流体柱液态染色学 (palmLC) 平台使得传统中医 (TCM) 分析的高效,低成本的静止相选成为可能. 这种方法显著提高了选效率,同时保持了色谱性能,以更好地控制质量.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 微流体学 微流体学
背景情况:
- 静止阶段查 (SPS) 对于药物研究中的染色分离至关重要,特别是在复杂的传统中医 (TCM) 中.
- 当前的SPS方法往往是低效的,昂贵的,并忽视静止相之间的微妙差异,如各种C18类型,影响TCM组件分离.
- 优化静止相选择对于实现高选择性和TCM中活性成分的准确分析至关重要.
研究的目的:
- 开发一种高效,低成本,易于原型化的平行阵列微流体柱液态染色学 (palmLC) 平台,用于静止相选.
- 与标准系统相比,评估palmLC平台在染色学效率和分辨率方面的性能.
- 证明palmLC平台的实用性,用于识别最佳静止阶段,用于分析复杂的TCM.
主要方法:
- 开发了一个palmLC平台,使用批量准备的毛细管柱和多通道毛细管微流体组件进行并行静止相选.
- 将palmLC系统的染色学性能 (效率和分辨率) 与标准单列液态染色学系统进行比较.
- 在分析*Panax notoginseng*,*Gastrodia elata*和*Polygala tenuifolia*时,应用了palmLC平台进行静止阶段选.
主要成果:
- 棕LC系统的染色学性能与标准系统相提并论 (例如,效率:5110与5150板;分辨率:2.77与3.39).
- 使用palmLC平台实现了600%的选效率提高.
- 成功确定了最佳的C18静止阶段,用于分离 *Panax notoginseng* 和 *Gastrodia elata* 中的目标组件,并为 *Polygala tenuifolia* *提供了全面的分离配置.
结论:
- 开发的palmLC平台为染色体分析中的静止相选提供了一种高效,具有成本效益和可靠的解决方案.
- 该平台有效地同时选多个静止相,为TCM等复杂样品的分离能力提供全景.
- 棕LC策略是传统中医分析中高清质量控制和分析的宝贵工具.
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
572
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
572
01:17Gas Chromatography: Types of Columns and Stationary Phases
381
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
381
01:05High-Performance Liquid Chromatography: Elution Process
320
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
320
00:57High-Performance Liquid Chromatography: Instrumentation
496
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
496
01:10Silica Gel Column Chromatography: Overview
840
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
840
01:13Principles Of Column Chromatography
6.5K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.5K