IFITM蛋白质是猪流行性腹冠状病毒的关键进入因素
Lilei Lv1, Huaye Luo1, Jingxuan Yi1,2
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Journal of virology
|May 12, 2025
概括
与IFITM3和IFITM1一样的干扰素诱导性跨膜 (IFITM) 蛋白质是促进猪流行性腹病毒 (PEDV) 进入和复制的关键宿主因素. 针对这些IFITM蛋白质提供了一个潜在的策略来控制猪业中PEDV的传播.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在全球猪业造成重大损失.
- 对参与PEDV感染的宿主因素的有限理解需要进一步的研究.
研究的目的:
- 为了确定介导PEDV进入和复制的宿主因素.
- 调查干扰素诱导性跨膜蛋白 (IFITM) 在PEDV感染中的作用.
主要方法:
- 在PEDV允许细胞系中的全基因组CRISPR/Cas9基因淘汰屏幕.
- 用人类和猪 IFITM 蛋白进行基因沉默,重新引入和过度表达实验.
- 共同免疫沉和局部化试验.
- 在猪小肠有机体中进行感染研究.
主要成果:
- 内源的IFITM3增强了PEDV的进入和复制;它的缺失抑制了感染.
- 人类IFITM3/IFITM2的过度表达,但不是IFITM1,增加了PEDV的入口.
- 猪IFITM1过度表达增强了PEDV感染,而IFITM2/3需要C端切断以获得类似的效果.
- 所有的IFITM都与PEDV S1蛋白相互作用,这表明它在病毒进入中发挥了直接作用.
结论:
- IFITM蛋白质是关键的宿主因素,促进PEDV的进入和复制.
- 人类IFITM3和猪IFITM1是PEDV感染机制的关键参与者.
- 针对IFITM提供了一种潜在的治疗策略,用于控制PEDV传播并减轻对猪产业的经济影响.
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