相关实验视频
Updated: May 13, 2025

08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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CircAge:跨物种和组织的与衰老相关的循环RNA综合资源
Xin Dong1, Zhen Zhou1,2, Yanan Wang1,2,3,4
1State Key Laboratory of Genetic Evolution & Animal Models, Key Laboratory of Healthy Aging Research of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650201, China.
Genomics, proteomics & bioinformatics
|May 12, 2025
概括
循环RNAs (circRNAs) 是稳定的分子,可以调节衰老. CircAge数据库整合了跨物种的与衰老相关的circRNA数据,有助于发现与年龄有关的疾病的生物标志物.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 衰老研究研究 衰老研究
背景情况:
- 循环RNAs (circRNAs) 是一种具有独特的循环结构和高稳定性的RNA分子.
- 新出现的证据表明,circRNAs在衰老过程中起着重要的调节作用.
- 目前缺乏一个系统的资源,整合与衰老相关的circRNA数据.
研究的目的:
- 开发一个全面的数据库,CircAge,用于与衰老相关的circRNAs.
- 系统地分析和整合与衰老相关的circRNA数据.
- 促进研究circRNAs在衰老中的调节机制.
主要方法:
- 收集和分析了来自7个物种和24种组织类型的803个与衰老相关的样本.
- 通过使用高通量测序,从小鼠和 rhesus 子产生了 47 个新的组织样本.
- 来自多个生物信息学工具的综合预测,以识别独特的circRNA及其相互作用.
主要成果:
- 确定了超过529,856个独特的circRNAs.
- 随着年龄的增长,观察到circRNA表达水平的一般增加.
- 发现大约23%的circRNAs表现出跨物种的序列保护.
- CircAge数据库预测circRNA-miRNA-RBP相互作用,并评估circRNA编码潜力.
结论:
- 该CircAge数据库提供了一个基本的资源,以了解circRNA调节在衰老.
- 这一资源将加速老龄化生物学研究和新生物标志物的发现.
- CircAge促进了对衰老和与年龄有关的疾病的诊断和治疗策略的开发.
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