Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Weight-Controllable Biobarcode Probes for Multi-input Breast Cancer Diagnosis.

Nano letters·2026
Same author

Reagent-Free Molecular Pendulum Biosensor with Antibody-Aptamer Dual Recognition for Protein Analysis.

ACS sensors·2026
Same author

CEMs-SELEX: DNAzyme-powered de novo biomarker discovery and noninvasive cancer diagnosis.

Science advances·2026
Same author

Proximity-Induced Phosphatase-Recruiting DNA Feedback Switch for Self-Adaptive Modulation of Receptor Signaling and Cell Behavior.

Journal of the American Chemical Society·2026
Same author

DNA Logic Circuit-Equipped Redox Imbalance Amplifier for Precise Mitochondrial Disruption and Efficient Cancer Therapy.

Analytical chemistry·2026
Same author

Adenosine Triphosphate-Producing Artificial Cells: Biomimetic Construction Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026

相关实验视频

Updated: May 9, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

9.4K

庞索S作为蛋白质液态-液态相分离的向调节器.

Can Liu1, Qingpeng Zeng1, Xiuqin Ju1

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China.

Analytical chemistry
|May 12, 2025
PubMed
概括

庞索S通过结合疏水区选择性地诱导和监测蛋白质液体-液体相分离 (LLPS). 这种可控制的方法为生物分子工程和药物输送应用提供了新的可能性.

更多相关视频

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.6K
Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.0K

相关实验视频

Last Updated: May 9, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

9.4K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.6K
Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.0K

科学领域:

  • 生物化学 生物化学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 液-液相分离 (LLPS) 对于细胞组织和形成生物分子凝结物至关重要.
  • 诱导蛋白质LLPS的现有方法往往缺乏对时间和空间的精确控制.

研究的目的:

  • 推出Ponceau S作为一种用于诱导和监测蛋白LLPS的新型选择性调节器.
  • 研究Ponceau S影响蛋白质相分离的机制.
  • 为了探索Ponceau S诱导的LLPS的捕捞能力.

主要方法:

  • 利用Ponceau S诱导和观察牛血清白蛋白和酶中的LLPS.
  • 分析了Ponceau S光变化在蛋白质丰富的阶段内推断分子运动.
  • 调整盐度和1,6-二醇水平以调节滴滴动态.

主要成果:

  • 庞索S通过与疏水区域相互作用,有效地促进了蛋白质复合物的形成,形成液滴.
  • 滴体内的Ponceau S光度增加表明分子运动受限,证实了相位分离.
  • 阶段分离动态,包括滴滴融合和溶解,可以通过盐和1,6-二醇度进行微调.

结论:

  • 庞索S作为蛋白质LLPS的选择性小分子调节器.
  • 庞索S可以精确调节和监控蛋白质相位分离.
  • 这种方法为生物分子工程,药物输送和合成生物学方面的进步提供了潜力.