通过重建 Bacillus subtilis 中的定数感应系统 ComQXPA 来动态调节伊图林的产生
Guojun Wu1, Chenyue Yin1, Jie Zheng1
1Key Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, P.R. China.
World journal of microbiology & biotechnology
|May 12, 2025
概括
通过设计其定数感应 (QS) 系统,Bacillus subtilis iturin的产量得到了显著提高. 修改促进剂和降低调节ComX降解酶的AprE和NprE提高了它的产量.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 定数感应 (QS) 对于细菌细菌生物膜的形成和二次代谢物生产至关重要.
- 费洛蒙ComX以细胞密度依赖的方式通过表面素促进剂 (Psrf) 调节表面素的产生.
- 伊图林是一种脂,是一种由 Bacillus subtilis 生产的关键生物活性分子.
研究的目的:
- 为了改造Bacillus subtilis以提高它的产量.
- 研究不同促进剂和ComX降解酶在伊图林生物合成中的作用.
- 开发一个动态的监管系统,以优化伊图林产量.
主要方法:
- 用构成性 (P43),QS (Psrf) 和突变的QS (PM-srf) 促进体替换原生Pitu促进体.
- 通过CRISPR干扰 (CRISPRi) 系统来降低ComX降解酶AprE和NprE的下调.
- 高性能液体染色学 (HPLC) 和超高性能液体染色学-高分辨率电喷射电离-质谱学 (UPLC-HRESI-MS/MS) 用于量化和识别.
主要成果:
- 而PM-srf促进菌株增加了2.15倍 (409.33 mg·L-1) 的伊图林产量.
- 确定了四种新的C10-C13伊图林.
- 对AprE和NprE的下调单独增加了它的产量,分别为526.46 mg·L-1和416.99 mg·L-1.
- 同时下调AprE和NprE导致3.05倍的增加 (579.85毫克·L-1).
结论:
- 在Bacillus subtilis中设计ComQXPA QS系统显著提高了iturin的产量.
- 动态调节ComX降解酶为优化生物活性分子产量提供了一个强大的策略.
- 这项研究为Bacillus subtilis的代谢工程提供了一个强大的平台,用于工业应用.
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