相关实验视频
Updated: May 14, 2025

06:11
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
14.7K
在哺乳动物脏中,Dmrt2和Hmx2通过对抗性和辅助性调节过程,直接影响干细胞多样性
Riana K Parvez1, Doh Kyung Kim1, Réka L Csipán1
1Department of Stem Cell and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033.
概括
转录调节器Dmrt2和Hmx2/3对于脏干细胞 (IC) 亚型的规范至关重要. 它们的受限表达和相互抑制的相互作用建立了不同的IC身份,通过二碳酸盐输送物Slc4a1和Slc26a4.4控制pH平衡.
科学领域:
- 脏生理学和细胞生物学
- 在发育中的转录调节.
- 离子运输和酸稳态.
背景情况:
- 脏间细胞 (ICs) 通过特定亚型的二碳酸盐载体维持循环pH值.
- 类型A-ICs (A-ICs) 使用Slc4a1来提高pH,而类型B-ICs (B-ICs) 使用Slc26a4来降低pH.
- 非A-非B ICs (nA/nB-ICs) 也表达Slc26a4,但它们的功能尚不清楚.
研究的目的:
- 阐明转录调节器Dmrt2,Hmx2和Hmx3在指定IC亚型中的作用.
- 调查IC亚型确定和转载基因表达的基础分子机制.
- 探索这些调节器与IC差异化中的泛决定因素Foxi1之间的相互作用.
主要方法:
- 在小鼠脏中的Dmrt2,Hmx2和Hmx3的CRISPR/Cas9介导基因淘汰.
- 在基因操纵后对IC亚型标记物表达 (Slc4a1,Slc26a4,Hmx2,Dmrt2) 的分析.
- 在含有或不含Foxi的尿道器官培养物中Hmx2和Dmrt2的异胎激活1.
主要成果:
- 移除Dmrt2诱导A-ICs采用B-IC命运 (Hmx2+/Slc26a4+). 由于Dmrt2的移除,A-ICs被诱导采用B-IC命运 (Hmx2+/Slc26a4+).
- 删除Hmx2/Hmx3诱导的B-ICs启动一个A-IC程序 (Dmrt2+/Slc4a1+).
- 三重淘汰导致混合IC表达Slc4a1和Slc26a4,表明这些监管机构的重要作用.
- 在有机体中,Foxi1激活了Dmrt2的A-IC发育,而Hmx2抑制了Foxi1依赖的Dmrt2并调节了Slc26a4.4.
- 对Foxi1和Dmrt2的同时表达上调Slc4a1.1.
结论:
- Dmrt2和Hmx2/3之间相互抑制的相互作用对于建立独特的IC身份至关重要.
- 这些转录调节器协调基因调节程序,定义特定的IC亚型.
- 了解这些机制可以了解脏的发育和pH调节.
相关概念视频
Renal Tubule and Collecting Duct
546
The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
546
Reabsorption and Secretion in the DCT and Collecting Duct
781
The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal...
The distal...
781
Renal Regulation of Acid-Base Balance
173
Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
173
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
Glomerular Filtration Rate and its Regulation
1.8K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
1.8K

