Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

335
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
335
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

538
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
538
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.1K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.1K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

653
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
653
Mass Spectrometers01:16

Mass Spectrometers

4.9K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
4.9K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

1.8K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
1.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ontogeny independent expression of LPCAT2 in granuloma macrophages during experimental visceral leishmaniasis.

Communications biology·2026
Same author

Dysregulated Lipid and Protein Networks in Osteochondrosis.

Journal of proteome research·2026
Same author

Efficient Compression of Mass Spectrometry Images via Contrastive Learning-Based Encoding.

Analytical chemistry·2025
Same author

Toward molecular phenotyping of temporal lobe epilepsy by spatial omics.

Epilepsia·2025
Same author

Spatial Distribution of Brain PET Tracers by MALDI Imaging.

Journal of the American Society for Mass Spectrometry·2025
Same author

Patient-responsive protein biomarkers for cartilage degeneration and repair identified in the infrapatellar fat pad.

Expert review of proteomics·2024

相关实验视频

Updated: May 14, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.3K

批量效应评估和纠正在质谱成像中的质量控制标准.

Luojiao Huang1, Yaejin Kim1, Benjamin Balluff2

  • 1Cell Biology-Inspired Tissue Engineering, Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6229ER Maastricht, Netherlands.

Analytical chemistry
|May 12, 2025
PubMed
概括

一个新的质量控制标准 (QCS) 和数据分析管道提高了矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 的可重复性. 这种方法解决了技术变异,以提高数据质量和可靠的空间分子分析.

更多相关视频

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

11.9K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

相关实验视频

Last Updated: May 14, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.3K
Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

11.9K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

科学领域:

  • 分析化学 分析化学
  • 生物技术是生物技术.
  • 质谱仪成像成像 质谱仪成像

背景情况:

  • 矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 能够进行空间分子分析,但在可重现性方面面临挑战.
  • 样本准备和仪器性能方面的技术差异阻碍了MALDI-MSI的准确评估和数据质量.
  • 需要一种标准化的质量控制和批量效应校正方法,以提高MALDI-MSI结果的可靠性.

研究的目的:

  • 开发和验证一个新的质量控制标准 (QCS) MALDI-MSI.
  • 引入一个数据分析管道,用于评估和纠正MALDI-MSI的技术变化.
  • 使用MALDI-MSI提高空间分子分析的可复制性和数据质量.

主要方法:

  • 设计了一种模仿组织的QCS,在凝基质中使用propranolol来模拟离子抑制.
  • 进行了为期三天的批量实验,以评估QCS对纵向技术变化的敏感性.
  • 应用了三种计算方法来对MALDI-MSI数据进行批量效应校正,包括主要组件分析 (PCA).

主要成果:

  • 开发的QCS有效地模仿了组织样本中观察到的离子抑制.
  • 质量控制系统表现出对纵向技术变化的敏感性,作为批量效应的有效指标.
  • 批量效应校正显著降低了QCS变化,并在PCA中改善了样本聚类,提高了数据质量.

结论:

  • 设计的QCS为MALDI-MSI中评估批量效应提供了可靠的工具.
  • 数据分析管道有效地纠正技术差异,改善MALDI-MSI数据的可复制性.
  • 这种综合方法为MALDI-MSI用户提供了一种可靠的质量控制方法和增强的数据分析.