使用CO气体感应转录ON/OFF模块对乙酸性细菌进行代谢工程
Sangrak Jin1, Irisappan Ganesh2, Jiyun Bae3
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea; KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea; Department of Biotechnology, Yeungnam University, Gyeongbuk, 38542, South Korea.
Metabolic engineering
|May 12, 2025
概括
研究人员开发了新型的一氧化碳 (CO) 生物传感器,用于乙酸性细菌. 这些传感器从二氧化碳中增强了诸如2,3-butanediol (2,3-BDO) 等有价值化学物质的产生,提高了生物制造的效率.
科学领域:
- 合成生物学 合成生物学
- 微生物生物技术 微生物生物技术
- 代谢工程是代谢工程.
背景情况:
- 开发用于微生物细胞中动态气体传感的生物传感器,特别是有毒一氧化碳 (CO),是一项挑战.
- 乙菌提供了将二氧化碳转化为附加值化学品的潜力,但需要精确的代谢控制.
研究的目的:
- 为Eubacterium limosum设计直角的CO感应转录调节模块 (开启/关闭).
- 为了利用这些模块来增强2,3-butanediol (2,3-BDO) 从乙原中的CO的生物合成.
主要方法:
- 集成CO结合转录激活器 (CooA,RcoM1) 与一个Halo-tag光传递器系统.
- 使用CRISPR/Cas9删除E. limosum中的乳酸路径以重定向碳流.
- 开发一个两阶段的CO-葡萄糖混合营养发酵策略.
主要成果:
- 成功开发了用于E. limosum的CO感应ON/OFF模块.
- 通过使用CO-ON模块激活目标基因,实现了2,3-BDO产量的1.7倍增加.
- 经过基因改造的菌株显示,在乳酸途径被删除后,用于生产2,3-BDO的碳利用率增加了18.5%.
- 使用优化菌株和发酵策略,生产了大约52g/L的2,3-BDO.
结论:
- 工程 CO 传感模块提供了有效的工具来控制乙原体中的代谢途径.
- 开发的平台可以有效地将二氧化碳转化为有价值的生物化学物质,如2,3-BDO.
- 这项工作推进了用于可持续化学品生产的乙基生物催化剂的工程.
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