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通过电穿孔评估脂质体纳米载体的向药物输送通过电穿孔

Silvia Pisani¹, Godspower Tochukwu Isaac¹, Rossella Dorati¹

¹Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.

International journal of pharmaceutics

|May 12, 2025

概括

带正电荷的胺硫酸盐加载脂质体与电穿孔 (EP) 结合,显著增强药物吸收和释放. 这种新的方法优化了细胞内药物度,以改善癌症治疗结果.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
药理学药理学是指药理学的学科.

背景情况:

电穿孔 (EP) 通过暂时增加细胞膜的透性来增强药物输送,在癌症治疗 (电化学疗法) 中具有最小毒性的优势.
在EP后增加细胞内药物吸收仍然是提高治疗疗效的挑战.
脂质体,由于其生物相容性和与细胞膜的结构相似性,是基于EP的药物输送的有希望的纳米载体.

研究的目的:

为了评估正电荷 gentamicin 硫酸盐加载脂质体 (GS-Lipo) 在通过电穿孔增强 gentamicin 硫酸盐吸收的有效性.
研究EP下脂质体的行为,包括药物释放和细胞内化.
优化EP参数以最大限度地提高细胞内药物度.

主要方法:

使用正电荷的胺素硫酸盐加载脂质体 (GS-Lipo) 进行药物输送.
使用不同的电压参数 (160 V,200 V,250 V) 应用电穿孔 (EP).
使用光脂质体和GS-Lipo进行体外研究,评估脂质体大小,PDI,药物释放和细胞内化.

主要成果:

电穿孔对脂质体大小 (低于250 nm) 和PDI的影响最小.
EP显著增强了细胞内吸收和药物释放,通过在脂质体双层中创建暂时的孔隙,促进 gentamicin 扩散.
与单个治疗相比,联合治疗 (EP + GS-Lipo) 在细胞吸收方面表现优越.

结论:

脂质体和电穿孔之间存在强烈的相互作用,增强药物输送.
优化的EP参数最大化了细胞内药物度,显示了改善治疗结果的潜力.
结合EP的GS-Lipo是一种有前途的策略,可以改善癌症治疗中的药物输送.

关键词:

电穿孔是一种电子穿孔.甘胺硫酸盐是什么甘胺硫酸盐脂质体是一种脂质体.微流体学微流体学

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